Nanoencapsulated bioactive esters: enzymatic synthesis and study of potential antimicrobial

• Grant number: 20/09592-1
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2021
• End date: December 31, 2023
• Field of knowledge: Engineering - Chemical Engineering - Chemical Process Industries
• Principal Investigator: Ariela Veloso de Paula
• Grantee: Ariela Veloso de Paula
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Daniela Remonatto ; Fernando Lucas Primo ; José Vladimir de Oliveira ; Juliana Cristina Bassan ; Rubens Monti

Abstract

The use of plants and extracts as therapies for the treatment of numerous diseases has been known since antiquity. Many of the beneficial health effects and performance in infectious treatments are due to the esters composition. The study of enzymatic synthesis esters, with antimicrobial capacity is fundamental importance, due the to search for natural compounds in substitution to the chemicals additive, usually found in foods. The objective of this study is the synthesis of geraniol esters, through enzymatic esterification using different reaction media, with the study of the antimicrobial activity. The study will be carried out using commercial lipases immobilized Novozym 435 and NS88011, in a batch reactor and packed bed reactor. As substrates will be used geraniol and citronella oil (Cymbopogon winterianus) and different acyl donors - cinnamic and acetic acids. The esters produced will be nanoencapsulated for nanoemulsions (NE) and polymeric nanocapsules (NC) methods, which have different release kinetics. Finally, the antimicrobial capacity will be verified, using the Kirby-Bauer agar diffusion method and the determination of the Minimum Inhibitory Concentration (MIC) of the free and encapsulated esters. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REMONATTO, DANIELA; FERRARI, BARBARA RIBEIRO; BASSAN, JULIANA CRISTINA; MUSSAGY, CASSAMO USSEMANE; SANTOS-EBINUMA, VALERIA DE CARVALHO; DE PAULA, ARIELA VELOSO. Utilization of Clay Materials as Support for Aspergillus japonicus Lipase: An Eco-Friendly Approach. CATALYSTS, v. 11, n. 10, OCT 2021. (19/15493-9, 20/08655-0, 18/06908-8, 20/09592-1, 17/11482-7)

REMONATTO, DANIELA; MIOTTI JR, RODNEY H.; MONTI, RUBENS; BASSAN, JULIANA C.; DE PAULA, ARIELA VELOSO. Applications of immobilized lipases in enzymatic reactors: A review. Process Biochemistry, v. 114, p. 20-pg., 2022-01-17. (20/09592-1)

DO AMARAL, STEPHANIE R.; AMANTINO, CAMILA F.; DE ANNUNZIO, SARAH R.; DE PAULA, ARIELA V.; FONTANA, CARLA R.; PRIMO, FERNANDO L.. Advanced methylene blue - nanoemulsions for in vitro photodynamic therapy on oral and cervical human carcinoma. Lasers in Medical Science, v. 37, n. 9, p. 8-pg., 2022-07-12. (20/09592-1)

REMONATTO, DANIELA; FANTATTO, RAFAELA REGINA; PIETRO, ROSEMEIRE CRISTINA LINHARI RODRIGUES; MONTI, RUBENS; OLIVEIRA, J. VLADIMIR; DE PAULA, ARIELA VELOSO; BASSAN, JULIANA CRISTINA. Enzymatic synthesis of geranyl acetate in batch and fed-batch reactors and evaluation of its larvicidal activity against Rhipicephalus (Boophilus) microplus. Process Biochemistry, v. 120, p. 14-pg., 2022-06-27. (20/09592-1)

BRUNA JEANNE SOARES PACHECO; GRAZIELLE SANTOS SILVA ANDRADE; ARIELA VELOSO DE PAULA. LIPASE FROM RHIZOPUS ORYZAE IMMOBILIZED ON CORN COB POWDER: A NEW APPROACH FOR DIETETIC TRIGLYCERIDE SYNTHESIS IN A FIXED BED REACTOR. Química Nova, v. 47, n. 7, 2024-03-29. (20/09592-1)

DOMINGUES, OTAVIO; REMONATTO, DANIELA; DOS SANTOS, LETICIA KAREN; MARTINEZ GALAN, JULIAN PAUL; FLUMIGNAN, DANILO LUIZ; DE PAULA, ARIELA VELOSO. Evaluation of Candida rugosa Lipase Immobilized on Magnetic Nanoparticles in Enzymatic/Chemical Hydroesterification for Biodiesel Production. Applied Biochemistry and Biotechnology, v. 194, n. 11, p. 24-pg., 2022-07-05. (18/09904-3, 20/09592-1)

REMONATTO, DANIELA; SANTAELLA, NUBIA; LERIN, LINDOMAR ALBERTO; BASSAN, JULIANA CRISTINA; CERRI, MARCEL OTAVIO; DE PAULA, ARIELA VELOSO. Solvent-Free Enzymatic Synthesis of Dietary Triacylglycerols from Cottonseed Oil in a Fluidized Bed Reactor. Molecules, v. 28, n. 14, p. 18-pg., 2023-07-01. (20/09592-1, 20/08699-7)

SOARES PACHECO, BRUNA JEANNE; DOMINGUES, OTAVIO; REINA, MARIANA PRADO; DE BABTISTA NETO, ALVARO; SILVA ANDRADE, GRAZIELLE SANTOS; DE PAULA, ARIELA VELOSO. Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers. Biotechnology Journal, v. 17, n. 4, p. 8-pg., 2022-02-01. (20/09592-1, 17/11482-7)