Characterization and standardization of chemical, physical and biological processes for the production of Lentinula edodes substrate.

• Grant number: 23/07948-1
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2024
• End date: January 31, 2026
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Diego Cunha Zied
• Grantee: Diego Cunha Zied
• Host Institution: Faculdade de Ciências Agrárias e Tecnológicas. Universidade Estadual Paulista (UNESP). Campus de Dracena. Dracena , SP, Brazil
• City of the host institution: Dracena
• Associated researchers: André Rodrigues ; Arturo Pardo Giménez ; Eustáquio Souza Dias

Abstract

The standardization of substrate production for mushroom cultivation in Brazil is a major challenge faced by fungiculture, mainly due to the great diversity of raw materials available in each region and the possibility of applying different methods of substrate preparation, involving chemical, physical and biological processes. The ideal substrate is one produced at a low cost, nutritionally balanced for the development and fruiting of the mushroom, physically adequate, allowing gas exchange and the formation of the mycelial browing, and biologically free of contaminants that could damage mushroom production. In this sense, the present project aims to standardize the methods of substrate production for the cultivation of Lentinula edodes. 6 different raw materials will be used, these being Brachiaria brizantha, Brachiaria decumbens, sugarcane bagasse, wheat straw, rice straw and sawdust, which were the object of a previous study and presented a high quality standard (FAPESP Process No. 2020/13230-8). All these substrates will be fermented (period of 5 days) or not and pasteurized (72 hours at 65°C) or sterilized (4 hours at 121°C). Two particle sizes (particles of 5 mm up to 15 mm) will be used. The substrates will be inoculated with two genetic strains, to obtain more accurate results. The chemical, physical and biological characteristics of the substrates, the agronomic behavior of L. edodes, the quality of the mushrooms and the production cost of the proposed systems will be evaluated. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

FREITAS, MARCOS ANTONIO DA SILVA; TEIXEIRA, PEDRO AFONSO GOMES; JUNIOR, WAGNER GONSALVES VIEIRA; PARDO-GIMENEZ, ARTURO; ZIED, DIEGO CUNHA. Liquid calcium supplementation between flushes increases shiitake yield. Scientia Horticulturae, v. 338, p. 7-pg., 2024-09-30. (20/13230-8, 23/07948-1)

VIEIRA JUNIOR, WAGNER GONCALVES; CAITANO, CINTHIA ELEN CARDOSO; ALVES, LUCAS DA SILVA; TEIXEIRA, PEDRO AFONSO GOMES; NOBLE, RALPH; PARDO, JOSE EMILIO; ZIED, DIEGO CUNHA. From waste to resource: Sustainable reuse of spent shiitake mushroom substrate in subsequent production cycles. INTERNATIONAL BIODETERIORATION & BIODEGRADATION, v. 200, p. 10-pg., 2025-02-27. (21/09034-1, 23/07948-1)

ROCHA, BRUNO DE SOUZA; VIEIRA JR, WAGNER GONCALVES; DE PAULA, ADRIANO TAFFAREL CAMARGO; SANTANA, ASSER BOTELHO; FREITAS, MARCOS ANTONIO DA SILVA; HIRAI, MILTON MINEO; ALVES, LUCAS DA SILVA; ZIED, DIEGO CUNHA. Impact of Substrate Amount and Fruiting Induction Methods in Lentinula edodes Cultivation. HORTICULTURAE, v. 11, n. 8, p. 12-pg., 2025-08-04. (23/07948-1)