Morphofunctional evaluation of the sublethal effects of microfibers of textile origin in association with the antibiotic amoxicillin in organisms of different trophic levels.

• Grant number: 23/02394-8
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2024
• End date: January 31, 2026
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Renata de Britto Mari
• Grantee: Renata de Britto Mari
• Host Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil
• Associated researchers: Caio Rodrigues Nobre ; Denis Moledo de Souza Abessa ; Marcos Antonio de Oliveira ; Paloma Kachel Gusso Choueri ; Rodrigo Brasil Choueri

Abstract

Microplastic pollution has been a growing concern in recent decades due to its impact on the environment. The adsorption capacity of the microplastic surface to various pollutants present in effluents, enhances the toxicity and dispersion capacity of these compounds in organisms at different levels of the trophic chain. Considering this problem, this work aims to evaluate the biochemical, cellular and histological alterations caused by plastic microfibers associated with the antibiotic amoxicillin in the gills, liver and intestine of the oyster biomodels Crassostrea brasiliana and two species of vertebrates: zebrafish Danio rerio and Wistar rats Rattus norvergicus. For this, the organisms will be exposed to treatments with nylon microfibers alone, amoxicillin alone and nylon microfibers fortified with amoxicillin, followed by a period of purification. After exposure, samples of gills, hepatopancreas, liver and intestine of organisms will be collected from each organism and changes in enzymes of the antioxidant system, lipid peroxidation (LPO), DNA damage (DNA strand-break) and histological damage will be evaluated. The data obtained will be statistically treated later, through the analysis of variance permutational (PERMANOVA) and analysis of principal components (PCA). It is expected that the results contribute to a better understanding of the mechanism of action and interaction between these contaminants and organisms of different trophic levels. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, JENNIFER BARBOSA; CHOUERI, RODRIGO BRASIL; DOS SANTOS, FRANCISCO EDUARDO MELO; SANTOS, LAIS ADRIELLE DE OLIVEIRA; DA SILVA, LETICIA FERNANDA; NOBRE, CAIO RODRIGUES; CARDOSO, MILTON ALEXANDRE; MARI, RENATA DE BRITTO; SIMOES, FABIO RUIZ; DELVALLS, TOMAS ANGEL; et al. Are Microfibers a Threat to Marine Invertebrates? A Sea Urchin Toxicity Assessment. TOXICS, v. 12, n. 10, p. 16-pg., 2024-10-01. (23/02394-8)