Advanced search
Start date

Evaluation of toxic effects after the exposure of reactive dyes extracted of cotton fibers with artificial sweat, using Salmonella mutagenicity and Comet assays and induction of cell death skin fibroblasts

Grant number: 10/05181-5
Support type:Regular Research Grants
Duration: May 01, 2010 - October 31, 2012
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:Danielle Palma de Oliveira
Grantee:Danielle Palma de Oliveira
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Assoc. researchers:Gisele Augusto Rodrigues de Oliveira


The fabrics dyeing began thousands of years ago and the commercial availability of dyes is increasing. The dyeing process is one of the key factors in the commercial success of textile products, since consumers are demanding products more resistant to exposure to heat, light, perspiration and washing. Because of that, nowadays, thousand of dyes are available for coloring proposes, especially for clothes. Nowadays these compounds are considered a problem regarding to public health due to their toxic, mutagenic and carcinogenic properties. According to the literature, conditions of intense perspiration generate a high rate of migration and subsequent penetration of textile dyes to human skin. This fact is relevant, since human exposure to this type of compound is extremely high. The dye molecular structure is divided in two parts: the cromophere group, responsible for the color and the part responsible for the fixation to the fiber. For reactive dyes, covalent bound between the fibers and the groups clorotriazin and sulfatoethilsulfone allow the fixation. We selected four reactive dyes: 3 with clorotriazin group and one with sulfatoethilsulfone. These dyes have different cromophere groups, such as azo, antraquinone and phtalocianin. In order to evaluate the toxic effects of the solution obtained after the extraction of cotton fiber dyed in the laboratory using artificial sweat as extractor, we will use Salmonella mutagenicity and Comet assays and induction of cell death in CALARG-17173SP, primate skin fibroblasts cells. (AU)