The natural dyes have been applied for the purpose of dyeing hair since ancient times. The discovery of para-phenylenediamine (PPD) in the nineteenth century enabled the development of industries in the manufacture of synthetic dyes with the same purpose. Hair may be colored by three mechanisms: I-temporary with basic dyes such as Basic Blue 41, Basic Red 76, Basic Yellow 57, Basic Brown 17, among others; II-Semi-permanent: they are the nitroaromatic dye (direct red 23), known as direct dyes, III-Permanent: The chemical process is involved in oxidative permanent hair dyes, using compounds such as para-phenylenediamine (PPD) and para-toluenediamine (PTD). This project aims to determine the IC50 of para-phenylenediamine (PPD) and the dyes Direct Red 23 and Basic Blue 41 in melanocytes, keratinocytes, fibroblasts, and melanomas (SKMel-9) and (SKMel-3) to further investigate the possible type of cell death and possible alterations in the cell cycle. Depending on the results presented, the dye will be applied artificial dermis and skin in an attempt to determine damages in a situation close to real. In addition, we will investigate the profile of mutagenicity of the dyes using the Salmonella assay and the Comet test using human keratinocytes.
News published in Agência FAPESP Newsletter about the scholarship: