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Microbiological, structural, mechanical and physical evaluation of resilient materials modified by the addition of antimicrobial agents for denture stomatitis treatment

Grant number: 10/07932-8
Support type:Regular Research Grants
Duration: October 01, 2010 - December 31, 2012
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Karin Hermana Neppelenbroek
Grantee:Karin Hermana Neppelenbroek
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

This study aims to evaluate microbiological, physical and mechanical properties of resilient materials after the addition of antimicrobial agents for denture stomatitis' treatment. The antimicrobial action of some agents (nystatin, miconazole, ketoconazole, itraconazole, and chlorhexidine) on the biofilm of Candida specimens (C. albicans, C. glabrata, and C. tropicalis) over resilient materials for temporary relining of prostheses (Softone and Trusoft) will be evaluated according to the determination of minimal inhibitory concentration (MIC) and sub-inhibitory concentration (MIC50). The fungal biofilm will be formed over specimens (with or without the drug addition) in aerobical conditions at 37ºC using YNB broth. The biofilm analyses will be made using two methods: tetrazolium salt reduction-XTT and confocal microscopy. MIC and MIC50 will be determined by the metabolic activity of viable cells according to XTT method after biofilm formation (48 h) and after 14 days. Confocal microscopy will be made on specimens presenting MIC and MIC50. After the determination of these concentrations, it will be analyzed the pattern of incorporation of those drugs in resilient specimens by using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) analyses. In addition, specimens with or without (control) drug addition will be submitted to Shore A hardness, superficial roughness, tensile strength, and peel bond strength to a denture base acrylic resin. These analyses will be made after the specimens have been immersed in distilled water at 37ºC for 24 h, 7, and 14 days. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NEPPELENBROEK, KARIN HERMANA; MELLO LIMA, JOZELY FRANCISCA; HOTTA, JULIANA; GALITESI, LUCAS LULO; POMPEIA FRAGA ALMEIDA, ANA LUCIA; URBAN, VANESSA MIGLIORINI. Effect of Incorporation of Antifungal Agents on the Ultimate Tensile Strength of Temporary Soft Denture Liners. JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY, v. 27, n. 2, p. 177-181, FEB 2018. Web of Science Citations: 2.
HOTTA, JULIANA; CRAL, WILSON GUSTAVO; SAKIMA, VINICIUS TATSUYUJI; LARA, VANESSA SOARES; URBAN, VANESSA MIGLIORINI; NEPPELENBROEK, KARIN HERMANA. Intraoral Device for Optimal Antifungal Delivery in a Rat Model. CURRENT DRUG DELIVERY, v. 14, n. 5, p. 658-667, 2017. Web of Science Citations: 2.
SANCHEZ-ALIAGA, ADELAIDA; GUIMARAES PELLISSARI, CLAUDIA VIVIANE; ARRAIS, CESAR AUGUSTO GALVA; MICHEL, MILTON DOMINGOS; NEPPELENBROEK, KARIN HERMANA; URBAN, VANESSA MIGLIORINI. Peel bond strength of soft lining materials with antifungal to a denture base acrylic resin. DENTAL MATERIALS JOURNAL, v. 35, n. 2, p. 194-203, APR 2016. Web of Science Citations: 3.
SILVA, MATHEUS JACOBINA; DE OLIVEIRA, DENISE G.; MARCILLO, OSCAR O.; NEPPELENBROEK, KARIN H.; LARA, VANESSA S.; PORTO, VINICIUS C. Effect of denture-coating composite on Candida albicans biofilm and surface degradation after disinfection protocol. INTERNATIONAL DENTAL JOURNAL, v. 66, n. 2, p. 86-92, APR 2016. Web of Science Citations: 2.
URBAN, VANESSA M.; LIMA, THIAGO F.; BUENO, MIRIAN G.; GIANNINI, MARCELO; ARIOLI FILHO, JOAO N.; DE ALMEIDA, ANA LUCIA P. F.; NEPPELENBROEK, KARIN H. Effect of the Addition of Antimicrobial Agents on Shore A Hardness and Roughness of Soft Lining Materials. JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY, v. 24, n. 3, p. 207-214, APR 2015. Web of Science Citations: 10.
BUENO, M. G.; URBAN, V. M.; BARBERIO, G. S.; DA SILVA, W. J.; PORTO, V. C.; PINTO, L. DE R.; NEPPELENBROEK, K. H. Effect of antimicrobial agents incorporated into resilient denture relines on the Candida albicans biofilm. ORAL DISEASES, v. 21, n. 1, p. 57-65, JAN 2015. Web of Science Citations: 24.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.