Validator laboratory for the human transcript validation initiative

• Grant number: 00/12490-2
• Support Opportunities: Genome Research Grants
• Start date: December 01, 2000
• End date: June 30, 2003
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Enilza Maria Espreafico
• Grantee: Enilza Maria Espreafico
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The aim of the project is to validate the structure and to define the sequences for at least 2,000 full-length human genes in a two-year period (216/validator laboratory). Gene sequences will be extracted from genomic sequence available through the Human Genome Project. Sequences will be used as a scaffold for EST (conventional and ORESTES) mapping as well as for gene prediction by computational analysis. Primers for validation of putative genes will be generated based on EST mapping, computational exon prediction and patterns of genomic organization, such a as average gene size, intron/exons size and distribution. Validation will be done mainly by RT-PCR, insert subcloning and sequencing. A databank for gene cataloguing wll be created and also will provide additional information as chromosomal location, expression pattern and alternative transcrits forms. (AU)