Recent acquisition of preparative high-performance liquid chromatograph for the purification and isolation of serine proteinases to produce fibrin sealant

• Grant number: 09/53846-9
• Support Opportunities: Multi-user Equipment Program
• Start date: August 01, 2010
• End date: July 31, 2012
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Benedito Barraviera
• Grantee: Benedito Barraviera
• Host Institution: Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP). Centro Virtual de Toxinologia. Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• City of the host institution: Botucatu
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

Fibrin sealant comprises two components: the first is a serine proteinase extracted from snake venom (Bothrops neuwiedi pauloensis or Crotalus durissus terrificus); and the second is a fibrinogen-rich cryoprecipitate extracted from the blood of buffaloes, cattle or sheep. These two components are combined at the time of application making up fibrin with adhesive properties. A similar product with medical and veterinary applicability has been imported and is described in the review article attached (1). Three projects in partnership have been developed. The first is dedicated to the study, standardization and structural and functional characterization of serine proteinase molecules extracted from snake venom. The second is concerned with the certification of animals, which have never consumed animal protein, to produce cryoprecipitate. The third is dedicated to its applicability in the experimental glueing of nerves in the presence or absence of stem cells. A major problem is that the sealant production is not enough to supply basic, clinical, and experimental research. In addition, the complementary projects will attempt to extract bioactive anti-snake venom components from medicinal plants for experimental tests in animals. The institutional and multidisciplinary projects will allow the definitive certification of the two components of the fibrin sealant. (AU)

Scientific publications

(23)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, ANA SILVIA S. B. S.; BARRAVIERA, BENEDITO; BARRAVIERA, SILVIA REGINA C. S.; ABBADE, LUCIANA P. F.; CARAMORI, CARLOS ANTONIO; FERREIRA, JR., RUI SEABRA. A success in Toxinology translational research in Brazil: Bridging the gap. Toxicon, v. 69, n. SI, p. 50-54, JUL 2013. (09/06280-0, 09/53846-9)

LOURENCO, JR., AIRTON; ZORZELLA CRESTE, CAMILA FERNANDA; DE BANOS, LUCIANA CURTOLO; DOS SANTOS, LUCILENE DELAZARI; PIMENTA, DANIEL C.; BARRAVIERA, BENEDITO; FERREIRA, JR., RUI SEABRA. Individual venom profiling of Crotalus durissus terrificus specimens from a geographically limited region: Crotamine assessment and captivity evaluation on the biological activities. Toxicon, v. 69, n. SI, p. 75-81, JUL 2013. (09/09774-3, 09/06280-0, 09/53846-9)

BISCOLA, NATALIA PERUSSI; CARTAROZZI, LUCIANA POLITTI; FERREIRA JUNIOR, RUI SEABRA; BARRAVIERA, BENEDITO; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE. Long-Standing Motor and Sensory Recovery following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair. NEURAL PLASTICITY, 2016. (12/08101-8, 14/06892-3, 11/23377-7, 11/23236-4, 09/53846-9)

ORSI, PATRICIA RODRIGUES; LANDIM-ALVARENGA, FERNANDA CRUZ; JUSTULIN, JR., LUIS ANTONIO; KANENO, RAMON; GOLIM, MARJORIE DE ASSIS; DOS SANTOS, DANIELA CARVALHO; ZORZELLA CRESTE, CAMILA FERNANDA; OBA, EUNICE; MAIA, LEANDRO; BARRAVIERA, BENEDITO; et al. A unique heterologous fibrin sealant (HFS) as a candidate biological scaffold for mesenchymal stem cells in osteoporotic rats. STEM CELL RESEARCH & THERAPY, v. 8, SEP 29 2017. (14/06001-1, 09/53846-9, 12/02689-3, 12/08101-8)

CARTAROZZI, LUCIANA P.; SPEJO, ALINE B.; FERREIRA, JR., RUI S.; BARRAVIERA, BENEDITO; DUEK, ELIANA; CARVALHO, JULIANA L.; GOES, ALFREDO M.; OLIVEIRA, ALEXANDRE L. R.. Mesenchymal stem cells engrafted in a fibrin scaffold stimulate Schwann cell reactivity and axonal regeneration following sciatic nerve tubulization. Brain Research Bulletin, v. 112, p. 14-24, MAR 2015. (14/06892-3, 12/08101-8, 11/23236-4, 09/53846-9)

BISCOLA, NATALIA PERUSSI; CARTAROZZI, LUCIANA POLITTI; ULIAN-BENITEZ, SUZANA; BARBIZAN, ROBERTA; CASTRO, MATEUS VIDIGAL; SPEJO, ALINE BARROSO; FERREIRA, JR., RUI SEABRA; BARRAVIERA, BENEDITO; RODRIGUES OLIVEIRA, ALEXANDRE LEITE. Multiple uses of fibrin sealant for nervous system treatment following injury and disease. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, n. 1, MAR 14 2017. (12/20456-6, 12/08101-8, 14/06892-3, 12/22750-9, 12/02689-3, 11/23377-7, 11/02889-0, 13/04409-0, 09/53846-9, 14/11405-4, 10/00729-2, 13/23030-2, 12/13514-0)

FERREIRA, JR., RUI SEABRA; DE BARROS, LUCIANA CURTOLO; FERNANDES ABBADE, LUCIANA PATIRCIA; CATHARINO SARTORI BARRAVIERA, SILVIA REGINA; CAVARIANI SILVARES, MARIA REGINA; DE PONTES, LETICIA GOMES; DOS SANTOS, LUCILENE DELAZARI; BARRAVIERA, BENEDITO. Heterologous fibrin sealant derived from snake venom: from bench to bedside - an overview. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, n. 1, APR 4 2017. (09/53846-9, 12/08101-8, 14/13299-7)

LOURENCO, AIRTON, JR.; ZORZELLA CRESTE, CAMILA FERNANDA; DE BANOS, LUCIANA CURTOLO; DOS SANTOS, LUCILENE DELAZARI; PIMENTA, DANIEL C.; BARRAVIERA, BENEDITO; FERREIRA, RUI SEABRA, JR.. Individual venom profiling of Crotalus durissus terrificus specimens from a geographically limited region: Crotamine assessment and captivity evaluation on the biological activities. Toxicon, v. 69, p. 7-pg., 2013-07-01. (09/09774-3, 09/06280-0, 09/53846-9)

CAMPAGNER, M. V.; BOSCO, S. M. G.; BAGAGLI, E.; CUNHA, M. L. R. S.; JERONIMO, B. C.; SAAD, E.; BISCOLA, N. P.; FERREIRA, R. S., JR.; BARRAVIERA, B.. MICROBIOLOGICAL EVALUATION OF DIFFERENT STRATEGIES FOR MANAGEMENT OF SNAKES IN CAPTIVITY. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 75, n. 16-17, p. 11-pg., 2012-01-01. (09/06280-0, 09/53846-9)

DA CUNHA, MARCELO RODRIGUES; MENEZES, FERNANDA ARAUJO; DOS SANTOS, GEOVANE RIBEIRO; LOPES PINTO, CLOVIS ANTONIO; BARRAVIERA, BENEDITO; AMARO MARTINS, VIRGINIA DA CONCEICAO; DE GUZZI PLEPIS, ANA MARIA; FERREIRA JUNIOR, RUI SEABRA. Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 18, n. 1, p. 196-203, JAN-FEB 2015. (12/08101-8, 09/06280-0, 12/02689-3, 09/53846-9)

BARBIZAN, ROBERTA; CASTRO, MATEUS V.; FERREIRA, JR., RUI SEABRA; BARRAVIERA, BENEDITO; OLIVEIRA, ALEXANDRE L. R.. Long-Term Spinal Ventral Root Reimplantation, but not Bone Marrow Mononuclear Cell Treatment, Positively Influences Ultrastructural Synapse Recovery and Motor Axonal Regrowth. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 15, n. 11, p. 19535-19551, NOV 2014. (14/06892-3, 12/08101-8, 11/23236-4, 09/53846-9)

CAMPAGNER, M. V.; BOSCO, S. M. G.; BAGAGLI, E.; CUNHA, M. L. R. S.; JERONIMO, B. C.; SAAD, E.; BISCOLA, N. P.; FERREIRA, JR., R. S.; BARRAVIERA, B.. MICROBIOLOGICAL EVALUATION OF DIFFERENT STRATEGIES FOR MANAGEMENT OF SNAKES IN CAPTIVITY. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 75, n. 16-17, SI, p. 1070-1080, 2012. (09/06280-0, 09/53846-9)

SAAD, EDUARDO; BARROS, LUCIANA CURTOLO; BISCOLA, NATALIA; PIMENTA, DANIEL C.; BARRAVIERA, SILVIA R. C. S.; BARRAVIERA, BENEDITO; FERREIRA, JR., RUI SEABRA. INTRASPECIFIC VARIATION OF BIOLOGICAL ACTIVITIES IN VENOMS FROM WILD AND CAPTIVE Bothrops jararaca. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 75, n. 16-17, SI, p. 1081-1090, 2012. (09/06280-0, 09/53846-9)

RUI SEABRA FERREIRA JR; LUCIANA CURTOLO DE BARROS; LUCIANA PATRÍCIA FERNANDES ABBADE; SILVIA REGINA CATHARINO SARTORI BARRAVIERA; MARIA REGINA CAVARIANI SILVARES; LETICIA GOMES DE PONTES; LUCILENE DELAZARI DOS SANTOS; BENEDITO BARRAVIERA. Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, 2017-07-06. (09/53846-9, 12/08101-8, 14/13299-7)

NATALIA PERUSSI BISCOLA; LUCIANA POLITTI CARTAROZZI; SUZANA ULIAN-BENITEZ; ROBERTA BARBIZAN; MATEUS VIDIGAL CASTRO; ALINE BARROSO SPEJO; RUI SEABRA FERREIRA JR.; BENEDITO BARRAVIERA; ALEXANDRE LEITE RODRIGUES OLIVEIRA. Multiple uses of fibrin sealant for nervous system treatment following injury and disease. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, 2017-04-27. (09/53846-9, 10/00729-2, 13/04409-0, 11/02889-0, 13/23030-2, 12/22750-9, 12/08101-8, 12/13514-0, 11/23377-7, 12/02689-3, 14/11405-4, 14/06892-3, 12/20456-6)

ANDREIA VIEIRA PEREIRA; GUSTAVO DE BARROS; ERIKA GRACIELLE PINTO; ANDRE GUSTAVO TEMPONE; RICARDO DE OLIVEIRA ORSI; LUCILENE DELAZARI DOS SANTOS; SUELI CALVI; RUI SEABRA FERREIRA JR; DANIEL CARVALHO PIMENTA; BENEDITO BARRAVIERA. Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 22, 2016-02-19. (11/23236-4, 09/53846-9)

FERREIRA, ANA SILVIA S. B. S.; BARRAVIERA, BENEDITO; BARRAVIERA, SILVIA REGINA C. S.; ABBADE, LUCIANA P. F.; CARAMORI, CARLOS ANTONIO; FERREIRA, RUI SEABRA, JR.. A success in Toxinology translational research in Brazil: Bridging the gap. Toxicon, v. 69, p. 5-pg., 2013-07-01. (09/06280-0, 09/53846-9)

BISCOLA, NATALIA PERUSSI; CARTAROZZI, LUCIANA POLITTI; FERREIRA JUNIOR, RUI SEABRA; BARRAVIERA, BENEDITO; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE. Long-Standing Motor and Sensory Recovery following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair. NEURAL PLASTICITY, v. 2016, p. 19-pg., 2016-01-01. (14/06892-3, 09/53846-9, 12/08101-8, 11/23236-4, 11/23377-7)

BARBIZAN, ROBERTA; CASTRO, MATEUS V.; FERREIRA, RUI SEABRA, JR.; BARRAVIERA, BENEDITO; OLIVEIRA, ALEXANDRE L. R.. Long-Term Spinal Ventral Root Reimplantation, but not Bone Marrow Mononuclear Cell Treatment, Positively Influences Ultrastructural Synapse Recovery and Motor Axonal Regrowth. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 15, n. 11, p. 17-pg., 2014-11-01. (14/06892-3, 09/53846-9, 12/08101-8, 11/23236-4)

DA CUNHA, MARCELO RODRIGUES; MENEZES, FERNANDA ARAUJO; DOS SANTOS, GEOVANE RIBEIRO; LOPES PINTO, CLOVIS ANTONIO; BARRAVIERA, BENEDITO; AMARO MARTINS, VIRGINIA DA CONCEICAO; DE GUZZI PLEPIS, ANA MARIA; FERREIRA JUNIOR, RUI SEABRA. Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 18, n. 1, p. 8-pg., 2015-01-01. (12/02689-3, 12/08101-8, 09/06280-0, 09/53846-9)

SAAD, EDUARDO; BARROS, LUCIANA CURTOLO; BISCOLA, NATALIA; PIMENTA, DANIEL C.; BARRAVIERA, SILVIA R. C. S.; BARRAVIERA, BENEDITO; FERREIRA, RUI SEABRA, JR.. INTRASPECIFIC VARIATION OF BIOLOGICAL ACTIVITIES IN VENOMS FROM WILD AND CAPTIVE Bothrops jararaca. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 75, n. 16-17, p. 10-pg., 2012-01-01. (09/06280-0, 09/53846-9)

DA SILVA, D. A. F.; BISCOLA, N. P.; DOS SANTOS, L. D.; SARTORI, M. M. P.; DENADAI, J. C.; DA SILVA, E. T.; DUCATTI, C.; BICUDO, S. D.; BARRAVIERA, B.; FERREIRA, JR., R. S.. Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS). VETERINARY JOURNAL, v. 217, p. 119-125, NOV 2016. (09/53846-9, 12/02689-3, 12/08101-8)

GASPAROTTO, VINICIUS P. O.; LANDIM-ALVARENGA, FERNANDA C.; OLIVEIRA, ALEXANDRE L. R.; SIMOES, GUSTAVO FERREIRA; LIMA-NETO, JOAO F.; BARRAVIERA, BENEDITO; FERREIRA, JR., RUI S.. A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells. STEM CELL RESEARCH & THERAPY, v. 5, JUN 10 2014. (12/08101-8, 09/06280-0, 09/53846-9)