| Grant number: | 11/07891-2 |
| Support Opportunities: | Regular Research Grants |
| Start date: | July 01, 2011 |
| End date: | June 30, 2013 |
| Field of knowledge: | Agronomical Sciences - Food Science and Technology - Food Science |
| Principal Investigator: | Andrezza Maria Fernandes |
| Grantee: | Andrezza Maria Fernandes |
| Host Institution: | Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil |
| Associated researchers: | Carlos Augusto Fernandes de Oliveira ; Ricardo Luiz Moro de Sousa |
Abstract
The aim of the project is to isolate and identify fungi, evaluate the toxigenic potential of Aspergillus spp. and Fusarium spp. isolates, detect and quantify aflatoxins (AF) B1, B2, G1, G2 and fumonisins (F) B1 e B2 in samples of cereal mixtures denominated "ração humana". It will be analyzed cereal mixtures ready to eat, acquired from companies which trade the products nationally by internet. Samples of 15 different marks will be evaluated monthly, for a period of seven months, adding up 105 samples in the end of experiment. The unit sample will be constituted for original package with minimum of 250 g. Fungi from Aspergillus genus will be classified to specie level by genetic sequencing. Mycotoxins determination will be performed by high performance liquid chromatography, using immunoaffinity columns to clean up samples, following the evaluation of the method by performing analyses in samples artificially contaminated with toxins. Results will allow the knowledge of the contamination of the cereals mixture by toxigenic fungi and mycotoxins, and the simultaneous occurrence of aflatoxins and fumonisins in the products. (AU)
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