System biology as experimental strategy for discovery of novel natural products in the fauna of venomous arthropods from São Paulo State

• Grant number: 11/51684-1
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• Start date: October 01, 2012
• End date: September 30, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Mario Sergio Palma
• Grantee: Mario Sergio Palma
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated scholarship(s): 14/05376-1 - Metabolomics analysis of bee brains (Apis mellifera) in processes of memory acquisition, BP.MS; 13/26451-9 - Bioprospecting and Structural Analysis of the Silk Proteins of Arthropods by a Proteomics Approach Using nanoLC-ESI-CID/ETD System, BP.PD; 13/13542-6 - A neuroproteomic approach of the brain from Africanized Apis mellifera submitted to the assay proboscis extension reflex, BP.DR

Abstract

The demand for novel natural products is an important source of inspiration for most of the active ingredients of drugs worldwide; thus, there is a growing interest for the use of analytical platforms for increasingly sophisticated quantitative and qualitative identification of natural compounds in a fast and efficient way. Among these technologies, the combination of high resolution liquid chromatography and mass spectrometry of multiple steps (LC-MS and MSn) is the most used one. It is known so far that the insect venoms are complex mixtures of biologically active compounds, but few Brazilian species were chemically characterized to date. The main objective of this project is the standardization conditions for the analysis of the metabolomic "footprinting", using an LC (DAD) - IT-TOF/MS, MSn and GC-EI/HRMS, building initially, a database composed of low molecular weight compounds, characteristic of Arthropod venomous secretions. The venoms of different species of venomous Arthropods will be analyzed under the same analytical conditions in which the databank (DB) compounds, and the results compared with the DB for identification of low molecular weight compounds from such venomous secretions. This project will also address the development of protocols for studies of "analytical scanning" of natural library of peptides present in the venomous secretion of the Arthropods, based on the use of micro-scale techniques of LC-IT-TOF/MS MSn. The peptides sequenced by the strategy will be summarized and submitted to studies of characterization of the secondary structure of these peptides by spectroscopic methods (CD and NMR), and also to assays of biological activities, such as pain, inflammation, antibiosis, neurotoxicity / anti-epilepsy. The interaction of these peptides with membrane- mimetic systems (planar membranes and vesicles of different types) will be addressed with the use of biophysical techniques. Thus, this project seeks to fulfill some of the main objectives of the project BIOprospecTA -FAPESP for bioprospecting the biodiversity in the of São Paulo State. (AU)

Scientific publications

(39)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; LAMPRECHT, GUENTHER; CHEN, WEI-QIANG; HEO, SEOK; HARDY, JOHN GEORGE; PRIEWALDER, HELGA; SCHEIBEL, THOMAS RAINER; PALMA, MARIO SERGIO; LUBEC, GERT. Structure and post-translational modifications of the web silk protein spidroin-1 from Nephila spiders. JOURNAL OF PROTEOMICS, v. 105, n. SI, p. 174-185, JUN 13 2014. (10/19051-6, 11/51684-1)

DIAS, NATHALIA BATISTA; DE SOUZA, BIBIANA MONSON; GOMES, PAULO CESAR; BRIGATTE, PATRICIA; PALMA, MARIO SERGIO. Peptidome profiling of venom from the social wasp Polybia paulista. Toxicon, v. 107, n. B, SI, p. 290-303, DEC 1 2015. (11/51684-1)

ALVARES, DAYANE S.; WILKE, NATALIA; RUGGIERO NETO, JOAO; FANANI, MARIA LAURA. The insertion of Polybia-MP1 peptide into phospholipid monolayers is regulated by its anionic nature and phase state. Chemistry and Physics of Lipids, v. 207, n. A, p. 38-48, OCT 2017. (11/51684-1, 12/08147-8, 11/11640-5, 15/25619-9, 15/01508-3, 15/25620-7)

BAPTISTA-SAIDEMBERG, N. B.; SAIDEMBERG, D. M.; RIBEIRO, R. A.; ARCURI, H. A.; PALMA, M. S.; CARNEIRO, E. M.. Agelaia MP-I: A peptide isolated from the venom of the social wasp, Agelaia pallipes pallipes, enhances insulin secretion in mice pancreatic islets. Toxicon, v. 60, n. 4, SI, p. 596-602, SEP 15 2012. (11/51684-1)

PEREZ-RIVEROL, AMILCAR; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; MUSACCHIO LASA, ALEXIS; PALMA, MARIO SERGIO; BROCHETTO-BRAGA, MARCIA REGINA. Wasp venomic: Unravelling the toxins arsenal of Polybia paulista venom and its potential pharmaceutical applications. JOURNAL OF PROTEOMICS, v. 161, p. 88-103, MAY 24 2017. (13/26451-9, 14/13936-7, 11/51684-1)

PRATAVIEIRA, MARCEL; DA SILVA MENEGASSO, ANALLY RIBEIRO; CAVIQUIOLI GARCIA, ANA MARIA; DOS SANTOS, DIEGO SIMOES; GOMES, PAULO CESAR; MALASPINA, OSMAR; PALMA, MARIO SERGIO. MALDI Imaging Analysis of Neuropeptides in the Africanized Honeybee (Apis mellifera) Brain: Effect of Ontogeny. JOURNAL OF PROTEOME RESEARCH, v. 13, n. 6, p. 3054-3064, JUN 2014. (11/51684-1)

DE SOUZA, BIBIANA MONSON; DOS SANTOS CABRERA, MARCIA PEREZ; GOMES, PAULO CESAR; DIAS, NATHALIA BAPTISTA; STABELI, RODRIGO GUERINO; LEITE, NATALIA BUENO; NETO, JOAO RUGGIERO; PALMA, MARIO SERGIO. Structure-activity relationship of mastoparan analogs: Effects of the number and positioning of Lys residues on secondary structure, interaction with membrane-mimetic systems and biological activity. Peptides, v. 72, p. 164-174, OCT 2015. (11/51684-1)

LEITE, NATALIA BUENO; AUFDERHORST-ROBERTS, ANDERS; PALMA, MARIO SERGIO; CONNELL, SIMON D.; RUGGIERO NETO, JOAO; BEALES, PAUL A.. PE and PS Lipids Synergistically Enhance Membrane Poration by a Peptide with Anticancer Properties. BIOPHYSICAL JOURNAL, v. 109, n. 5, p. 936-947, SEP 1 2015. (11/11640-5, 11/51684-1)

ROAT, T. C.; DOS SANTOS-PINTO, J. R. A.; DOS SANTOS, L. D.; SANTOS, K. S.; MALASPINA, O.; PALMA, M. S.. Modification of the brain proteome of Africanized honeybees (Apis mellifera) exposed to a subaEurolethal doses of the insecticide fipronil. ECOTOXICOLOGY, v. 23, n. 9, p. 1659-1670, NOV 2014. (11/51684-1, 12/13370-8, 08/05018-7)

DA SILVA, ALESSANDRA. V. R.; DE SOUZA, BIBIANA M.; DOS SANTOS CABRERA, MARCIA P.; DIAS, NATHALIA B.; GOMES, PAULO C.; RUGGIERO NETO, JOAO; STABELI, RODRIGO G.; PALMA, MARIO S.. The effects of the C-terminal amidation of mastoparans on their biological actions and interactions with membrane-mimetic systems. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1838, n. 10, p. 2357-2368, OCT 2014. (04/07942-2, 11/51684-1)

MOURELLE, D.; BRIGATTE, P.; BRINGANTI, L. D. B.; DE SOUZA, B. M.; ARCURI, H. A.; GOMES, P. C.; BAPTISTA-SAIDEMBERG, N. B.; RUGGIERO NETO, J.; PALMA, M. S.. Hyperalgesic and edematogenic effects of Secapin-2, a peptide isolated from Africanized honeybee (Apis mellifera) venom. Peptides, v. 59, p. 42-52, SEP 2014. (04/07942-2, 11/51684-1)

DOS SANTOS CABRERA, MARCIA PEREZ; BALDISSERA, GISELE; SILVA-GONCALVES, LAIZ DA COSTA; DE SOUZA, BIBIANA MONSON; RISKE, KARIN A.; PALMA, MARIO SERGIO; RUGGIERO, JOSE ROBERTO; ARCISIO-MIRANDA, MANOEL. Combining Experimental Evidence and Molecular Dynamic Simulations To Understand the Mechanism of Action of the Antimicrobial Octapeptide Jelleine-I. BIOCHEMISTRY, v. 53, n. 29, p. 4857-4868, JUL 29 2014. (06/57122-7, 10/11823-0, 10/52077-9, 12/24259-0, 11/51684-1)

DECIO, PAMELA; VIEIRA, ALEXSANDRO SANTANA; DIAS, NATHALIA BAPTISTA; PALMA, MARIO SERGIO; BUENO, ODAIR CORREA. The Postpharyngeal Gland: Specialized Organ for Lipid Nutrition in Leaf-Cutting Ants. PLoS One, v. 11, n. 5, MAY 5 2016. (12/12541-3, 11/51684-1)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ARCURI, HELEN ANDRADE; ESTEVES, FRANCIELE GREGO; PALMA, MARIO SERGIO; LUBEC, GERT. Spider silk proteome provides insight into the structural characterization of Nephila clavipes flagelliform spidroin. SCIENTIFIC REPORTS, v. 8, OCT 2 2018. (16/16212-5, 10/19051-6, 13/26451-9, 11/51684-1, 15/14220-8)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ARCURI, HELEN ANDRADE; LUBEC, GERT; PALMA, MARIO SERGIO. Structural characterization of the major ampullate silk spidroin-2 protein produced by the spider Nephila clavipes. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1864, n. 10, p. 1444-1454, OCT 2016. (10/19051-6, 13/26451-9, 11/51684-1)

DOS SANTOS, LUCILENE DELAZARI; APARECIDO DOS SANTOS PINTO, JOSE ROBERTO; DA SILVA MENEGASSO, ANALLY RIBEIRO; SAIDEMBERG, DANIEL MENEZES; CAVIQUIOLI GARCIA, ANA MARIA; PALMA, MARIO SERGIO. Proteomic profiling of the molecular targets of interactions of the mastoparan peptide Protopolybia MP-III at the level of endosomal membranes from rat mast cells. PROTEOMICS, v. 12, n. 17, p. 12-pg., 2012-08-01. (11/51684-1)

ALVARES, DAYANE S.; WILKE, NATALIA; RUGGIERO NETO, JOAO; FANANI, MARIA LAURA. The insertion of Polybia-MP1 peptide into phospholipid monolayers is regulated by its anionic nature and phase state. Chemistry and Physics of Lipids, v. 207, p. 11-pg., 2017-10-01. (15/25620-7, 12/08147-8, 11/11640-5, 11/51684-1, 15/01508-3, 15/25619-9)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; LAMPRECHT, GUENTHER; CHEN, WEI-QIANG; HEO, SEOK; HARDY, JOHN GEORGE; PRIEWALDER, HELGA; SCHEIBEL, THOMAS RAINER; PALMA, MARIO SERGIO; LUBEC, GERT. Structure and post-translational modifications of the web silk protein spidroin-1 from Nephila spiders. JOURNAL OF PROTEOMICS, v. 105, p. 12-pg., 2014-06-13. (11/51684-1, 10/19051-6)

DA SILVA MENEGASSO, ANALLY RIBEIRO; PRATAVIEIRA, MARCEL; DA GAMA FISCHER, JULIANA DE SALDANHA; CARVALHO, PAULO COSTA; ROAT, THAISA CRISTINA; MALASPINA, OSMAR; PALMA, MARIO SERGIO. Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex. JOURNAL OF PROTEOMICS, v. 151, p. 14-pg., 2017-01-16. (11/51684-1)

DIAS, NATHALIA BAPTISTA; DE SOUZA, BIBIANA MONSON; COCCHI, FERNANDO KAMIMURA; CHALKIDIS, HIPOCRATES M.; CORONADO DORCEC, VALQUIRIA ABRAO; PALMA, MARIO SERGIO. Profiling the short, linear, non-disulfide bond-containing peptidome from the venom of the scorpion Tityus obscurus. JOURNAL OF PROTEOMICS, v. 170, p. 70-79, JAN 6 2018. (11/51684-1)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; CAVIQUIOLI GARCIA, ANA MARIA; ARCURI, HELEN ANDRADE; ESTEVES, FRANCIELE GREGO; SALLES, HELIANA CLARA; LUBEC, GERT; PALMA, MARIO SERGIO. Silkomics: Insight into the Silk Spinning Process of Spiders. JOURNAL OF PROTEOME RESEARCH, v. 15, n. 4, p. 1179-1193, APR 2016. (10/19051-6, 13/26451-9, 11/51684-1)

PEREZ-RIVEROL, AMILCAR; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; MUSACCHIO LASA, ALEXIS; PALMA, MARIO SERGIO; BROCHETTO-BRAGA, MARCIA REGINA. Wasp venomic: Unravelling the toxins arsenal of Polybia paulista venom and its potential pharmaceutical applications. JOURNAL OF PROTEOMICS, v. 161, p. 16-pg., 2017-05-24. (11/51684-1, 14/13936-7, 13/26451-9)

DOS SANTOS, LUCILENE DELAZARI; APARECIDO DOS SANTOS PINTO, JOSE ROBERTO; DA SILVA MENEGASSO, ANALLY RIBEIRO; SAIDEMBERG, DANIEL MENEZES; CAVIQUIOLI GARCIA, ANA MARIA; PALMA, MARIO SERGIO. Proteomic profiling of the molecular targets of interactions of the mastoparan peptide Protopolybia MP-III at the level of endosomal membranes from rat mast cells. PROTEOMICS, v. 12, n. 17, SI, p. 2682-2693, AUG 2012. (11/51684-1)

ESTEVES, FRANCIELE GREGO; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; SAIDEMBERG, DANIEL MENEZES; PALMA, MARIO SERGIO. Using a proteometabolomic approach to investigate the role of Dufour's gland in pheromone biosynthesis in the social wasp Polybia paulista. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 122-130, JAN 16 2017. (11/51684-1)

DA SILVA MENEGASSO, ANALLY RIBEIRO; PRATAVIEIRA, MARCEL; DA GAMA FISCHER, JULIANA DE SALDANHA; CARVALHO, PAULO COSTA; ROAT, THAISA CRISTINA; MALASPINA, OSMAR; PALMA, MARIO SERGIO. Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex. JOURNAL OF PROTEOMICS, v. 151, n. SI, p. 131-144, JAN 16 2017. (11/51684-1)

ARCURI, HELEN ANDRADE; GOMES, PAULO CESAR; DE SOUZA, BIBIANA MONSON; DIAS, NATHALIA BAPTISTA; BRIGATTE, PATRICIA; STABELI, RODRIGO GUERINO; PALMA, MARIO SERGIO. Paulistine-The Functional Duality of a Wasp Venom Peptide Toxin. TOXINS, v. 8, n. 3, MAR 2016. (11/51684-1)

ALVARES, DAYANE S.; RUGGIERO NETO, JOAO; AMBROGGIO, ERNESTO E.. Phosphatidylserine lipids and membrane order precisely regulate the activity of Polybia-MP1 peptide. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1859, n. 6, p. 1067-1074, JUN 2017. (12/08147-8, 15/25619-9, 11/11640-5, 15/25620-7, 11/51684-1, 15/01508-3)

BATISTA, ANDREA N. L.; DOS SANTOS-PINTO, JOSE ROBERTO A.; BATISTA, JR., JOAO M.; SOUZA-MOREIRA, TATIANA M.; SANTONI, MARIANA M.; ZANELLI, CLESLEI F.; KATO, MASSUO J.; LOPEZ, SILVIA N.; PALMA, MARIO S.; FURLAN, MAYSA. The Combined Use of Proteomics and Transcriptomics Reveals a Complex Secondary Metabolite Network in Peperomia obtusifolia. Journal of Natural Products, v. 80, n. 5, p. 1275-1286, MAY 2017. (13/07600-3, 11/51684-1, 14/25222-9, 11/01003-8)

GOMES, PAULO CESAR; DE SOUZA, BIBIANA MONSON; DIAS, NATHALIA BAPTISTA; BRIGATTE, PATRICIA; MOURELLE, DANILO; ARCURI, HELEN ANDRADE; DOS SANTOS CABRERA, MARCIA PEREZ; STABELI, RODRIGO GUERIN; RUGGIERO NETO, JOAO; PALMA, MARIO SERGIO. Structure-function relationships of the peptide Paulistine: A novel toxin from the venom of the social wasp Polybia paulista. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1840, n. 1, p. 170-183, JAN 2014. (11/51684-1)

DIAS, NATHALIA BAPTISTA; DE SOUZA, BIBIANA MONSON; GOMES, PAULO CESAR; PALMA, MARIO SERGIO. Peptide diversity in the venom of the social wasp Polybia paulista (Hymenoptera): A comparison of the intra- and inter-colony compositions. Peptides, v. 51, p. 122-130, JAN 2014. (09/11155-0, 11/51684-1)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ARCURI, HELEN ANDRADE; PRIEWALDER, HELGA; SALLES, HELIANA CLARA; PALMA, MARIO SERGIO; LUBEC, GERT. Structural Model for the Spider Silk Protein Spidroin-1. JOURNAL OF PROTEOME RESEARCH, v. 14, n. 9, p. 3859-3870, SEP 2015. (10/19051-6, 13/26451-9, 11/51684-1)

APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; DOS SANTOS, LUCILENE DELAZARI; ARCURI, HELEN ANDRADE; CASTRO, FABIO MORATO; KALIL, JORGE ELIAS; PALMA, MARIO SERGIO. Using Proteomic Strategies for Sequencing and Post-Translational Modifications Assignment of Antigen-5, a Major Allergen from the Venom of the Social Wasp Polybia paulista. JOURNAL OF PROTEOME RESEARCH, v. 13, n. 2, p. 855-865, FEB 2014. (11/51684-1)

SANTOS, KEITY SOUZA; STEPHANO, MARCO ANTONIO; MARCELINO, JOSE ROBERTO; RESENDE FERREIRA, VIRGINIA MARIA; ROCHA, THALITA; CARICATI, CELSO; HIGASHI, HISAKO GONDO; MORO, ANA MARIA; KALIL, JORGE ELIAS; MALASPINA, OSMAR; et al. Production of the First Effective Hyperimmune Equine Serum Antivenom against Africanized Bees. PLoS One, v. 8, n. 11, NOV 13 2013. (11/51684-1)

DOS SANTOS PINTO, JOSE R. A.; FOX, EDUARDO G. P.; SAIDEMBERG, DANIEL M.; SANTOS, LUCILENE D.; DA SILVA MENEGASSO, ANALLY R.; COSTA-MANSO, ELIUDE; MACHADO, EDNILDO A.; BUENO, ODAIR C.; PALMA, MARIO S.. Proteomic View of the Venom from the Fire Ant Solenopsis invicta Buren. JOURNAL OF PROTEOME RESEARCH, v. 11, n. 9, p. 4643-4653, SEP 2012. (11/51684-1, 06/02115-6)

APARECIDO DOS SANTOS PINTO, JOSE ROBERTO; DOS SANTOS, LUCILENE DELAZARI; ARCURI, HELEN ANDRADE; DIAS, NATHALIA BAPTISTA; PALMA, MARIO SERGIO. Proteomic Characterization of the Hyaluronidase (EC 3.2.1.35) from the Venom of the Social Wasp Polybia paulista. PROTEIN AND PEPTIDE LETTERS, v. 19, n. 6, p. 625-635, JUN 2012. (11/51684-1)

PEREZ-RIVEROL, AMILCAR; ROMANI FERNANDES, LUIS GUSTAVO; MUSACCHIO LASA, ALEXIS; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ABRAM, DEBORA MOITINHO; IZUKA MORAES, GABRIEL HIDEKI; JABS, FREDERIC; MIEHE, MICHAELA; SEISMMAN, HENNING; PALMA, MARIO SERGIO; et al. Phospholipase A1-based cross-reactivity among venoms of clinically relevant Hymenoptera from Neotropical and temperate regions. Molecular Immunology, v. 93, p. 87-93, JAN 2018. (13/26451-9, 14/13936-7, 11/51684-1)

ESTEVES, FRANCIELE GREGO; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; SAIDEMBERG, DANIEL MENEZES; PALMA, MARIO SERGIO. Using a proteometabolomic approach to investigate the role of Dufour's gland in pheromone biosynthesis in the social wasp Polybia paulista. JOURNAL OF PROTEOMICS, v. 151, p. 9-pg., 2017-01-16. (11/51684-1)

DIAS, NATHALIA BATISTA; DE SOUZA, BIBIANA MONSON; GOMES, PAULO CESAR; BRIGATTE, PATRICIA; PALMA, MARIO SERGIO. Peptidome profiling of venom from the social wasp Polybia paulista. Toxicon, v. 107, p. 14-pg., 2015-12-01. (11/51684-1)

BAPTISTA-SAIDEMBERG, N. B.; SAIDEMBERG, D. M.; RIBEIRO, R. A.; ARCURI, H. A.; PALMA, M. S.; CARNEIRO, E. M.. Agelaia MP-I: A peptide isolated from the venom of the social wasp, Agelaia pallipes pallipes, enhances insulin secretion in mice pancreatic islets. Toxicon, v. 60, n. 4, p. 7-pg., 2012-09-15. (11/51684-1)