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Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors

Abstract

Background: Cardiovascular diseases remain leaders as the major causes of mortality in Western society. Restoration of thecirculation through construction of bypass surgical treatment is regarded as the gold standard treatment of peripheralvascular diseases, and grafts are necessary for this purpose. The great saphenous vein is often not available and syntheticgrafts have their limitations. Therefore, new techniques to produce alternative grafts have been developed and, in thissense, tissue engineering is a promising alternative to provide biocompatible grafts. This study objective was to reconstructthe endothelium layer of decellularized vein scaffolds, using mesenchymal stem cells (MSC)s and growth factors obtained from platelets.Methods: Fifteen nonpregnant female adult rabbits were used for all experiments. Adipose tissue and vena cava were obtained and subjected to MSCs isolation and tissue decellularization, respectively. MSCs were subjected to differentiation using endothelial inductor growth factor (EIGF) obtained from human platelet lysates. Immunofluorescence,histological and immunohistochemical analyses were employed for the final characterization of the obtained blood vessel substitute.Results: The scaffolds were successfully decellularized with sodium dodecyl sulfate.MSCs actively adhered at the scaffolds, and through stimulation with EIGF were differentiated into functional endothelial cells, secreting significantly higher quantities of von Willebrand factor (0.85 mg/mL; P < .05) than cells cultivated under the same conditions, without EIGF(0.085 mg/mL). Cells with evident morphologic characteristics of endothelium were seen at the lumen of the scaffolds. These cells also stained positive for fascin protein, which is highly expressed by differentiated endothelial cells.Conclusions: Taken together, the use of decellularized bioscaffold and subcutaneous MSCs seems to be a potential approach to obtain bioengineered blood vessels, in the presence of EIGF supplementation. (J Vasc Surg 2013;-:1-9.) (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BERTANHA, MATHEUS; MOROZ, ANDREI; ALMEIDA, RODRIGO; ALVES, FLAVIA CILENE; VALERIO, MICHELE JANEGITZ ACORCI; MOURA, REGINA; DOMINGUES, MARIA APARECIDA CUSTODIO; SOBREIRA, MARCONE LIMA; DEFFUNE, ELENICE. Tissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factors. JOURNAL OF VASCULAR SURGERY, v. 59, n. 6, p. 1677-1685, JUN 2014. Web of Science Citations: 10.

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