Diagnostic strains of the Salmonella / microsome assay as a potential tool in effect directed analysis: application in a solution-oriented project

• Grant number: 13/16956-6
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2014
• End date: April 30, 2016
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Gisela de Aragão Umbuzeiro
• Grantee: Gisela de Aragão Umbuzeiro
• Host Institution: Faculdade de Tecnologia (FT). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated researchers: Fábio Kummrow

Abstract

Mutagenic chemicals are generally present in the environment as complex mixtures. Mutations can occur both in germ or somatic cells leading to several adverse effects. The analysis of previously known mutagenic compounds is not enough to identify all the mutagenic compounds present in environmental samples. Bioassays, like the Salmonella/microsome assay (Ames test), are key elements in an Effect Directed Analysis (EDA) because they can drive the chemical identification of priority pollutants. This project aims to use specific strains of Salmonella as diagnostic tools in the assay to help identify major mutagenic toxicants in the aquatic environment. The proposed project will be part of a collaborative solution-oriented project already approved by the European Commission -EU Work Programme 2013 named "Solutions for present and future emerging pollutants in land and water resources management". The specificities of the different Salmonella tester strains will be exploited to suggest the types or classes of mutagenic chemicals that are present in environmental sample extracts, and chemical analysis will be performed for confirmation of the results. If successful, this approach will be incorporated as a new diagnostic tool in EDA. We intend to identify new mutagenic compounds using the chemical and biological tools proposed in the Project, supporting the selection of priority pollutants in an integrated risk based approach. The involvement of the Brazilian participants in this project is a great opportunity and will certainly greatly increase the knowledge and expertise of the group, not only in the scientific issues, but also how to work with an international team focused on solutions to complex environmental problems. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALTENBURGER, ROLF; BRACK, WERNER; BURGESS, ROBERT M.; BUSCH, WIBKE; ESCHER, BEATE I.; FOCKS, ANDREAS; HEWITT, L. MARK; JACOBSEN, BO N.; LOPEZ DE ALDA, MIREN; AIT-AISSA, SELIM; et al. Future water quality monitoring: improving the balance between exposure and toxicity assessments of real-world pollutant mixtures. ENVIRONMENTAL SCIENCES EUROPE, v. 31, FEB 19 2019. (15/24758-5, 13/16956-6)

ZWARG, JOSE RICARDO R. M.; MORALES, DANIEL A.; MASELLI, BIANCA S.; BRACK, WERNER; UMBUZEIRO, GISELA A.. Miniaturization of the microsuspension Salmonella/microsome assay in agar microplates. Environmental and Molecular Mutagenesis, v. 59, n. 6, p. 488-501, JUL 2018. (15/24758-5, 13/16956-6, 15/11399-7)

NEALE, PETA A.; ALTENBURGER, ROLF; AIT-AISSA, SELIM; BRION, FRANCOIS; BUSCH, WIBKE; UMBUZEIRO, GISELA DE ARAGAO; DENISON, MICHAEL S.; DU PASQUIER, DAVID; HILSCHEROVA, KLARA; HOLLERT, HENNER; et al. Development of a bioanalytical test battery for water quality monitoring: Fingerprinting identified micropollutants and their Contribution to effects in surface water. WATER RESEARCH, v. 123, p. 734-750, OCT 15 2017. (15/24758-5, 13/16956-6)

BRACK, WERNER; AIT AISSA, SELIM; BACKHAUS, THOMAS; DULIO, VALERIA; ESCHER, BEATE I.; FAUST, MICHAEL; HILSCHEROVA, KLARA; HOLLENDER, JULIANE; HOLLERT, HENNER; MUELLER, CHRISTIN; et al. Effect-based methods are key. The European Collaborative Project SOLUTIONS recommends integrating effect-based methods for diagnosis and monitoring of water quality. ENVIRONMENTAL SCIENCES EUROPE, v. 31, p. 6-pg., 2019-02-08. (15/24758-5, 13/16956-6)

ALTENBURGER, ROLF; BRACK, WERNER; BURGESS, ROBERT M.; BUSCH, WIBKE; ESCHER, BEATE I.; FOCKS, ANDREAS; HEWITT, L. MARK; JACOBSEN, BO N.; LOPEZ DE ALDA, MIREN; AIT-AISSA, SELIM; et al. Future water quality monitoring: improving the balance between exposure and toxicity assessments of real-world pollutant mixtures. ENVIRONMENTAL SCIENCES EUROPE, v. 31, p. 17-pg., 2019-02-19. (15/24758-5, 13/16956-6)

BRACK, WERNER; AISSA, SELIM AIT; BACKHAUS, THOMAS; DULIO, VALERIA; ESCHER, BEATE I.; FAUST, MICHAEL; HILSCHEROVA, KLARA; HOLLENDER, JULIANE; HOLLERT, HENNER; MUELLER, CHRISTIN; et al. Effect-based methods are key. The European Collaborative Project SOLUTIONS recommends integrating effect-based methods for diagnosis and monitoring of water quality. ENVIRONMENTAL SCIENCES EUROPE, v. 31, FEB 8 2019. (15/24758-5, 13/16956-6)