Bone healing following Mid-Femur fracture in diabetic rats, with and without insulin treatment and sodium Pamidronate and PTH therapy

• Grant number: 13/12445-7
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2014
• End date: March 31, 2017
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: José Batista Volpon
• Grantee: José Batista Volpon
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Ariane Zamarioli

Abstract

Femoral bone tissue quality and post-fracture callus will be assessed in rats with diabetes induced by streptozotocin. Rats will be treated or not by insulin and sodium pamidronate and parathyroid hormone (PTH) and randomly assessed to 11 groups: (1) diabetic rats; (2) diabetic rats under control by insulin; (3) control rats submitted to bone fracture; (4) diabetic rats submitted to bone fracture; (5) diabetic rats controlled by insulin and submitted to bone fracture; (6) control rats treated with insulin and sodium alendronate; (7) diabetic rats treated with insulin and sodium pamidronate; (8) diabetic rats submitted to bone fracture and treated with insulin and sodium pamidronate; (9) control rats treated with insulin and PTH; (10) diabetic rats treated with insulin and PTH and (11) diabetic rats submitted to bone fracture and treated with insulin and PTH. In all groups, bones will be evaluated on days 14 and 28 post-fracture. Closed bone fracture will be created at the mid-femur with implant fixation inserted through the knee. Both bone callus and the non-fractured femur quality will be assessed using optical microscopy (tissue characterization), micro-tomography (qualitative and quantitative microstructure analysis), immunohistochemistry (bone formation and resorption evaluation: by means of dosage of osteocalcin, osteoprogeterin and RANK), bone mineral density analysis (DXA) and bone strength (mechanical test). In addition to that, adipokines will be assessed in order to investigate the role of bone on the endocrinological metabolism and, the systemic analysis of bone turnover markers (osteocalcin, osteoproeterin and RANK) Results will search the bone fracture and healing process, as well will evaluate the efficacy of blood level glucose control and pamidronate and PTH administrations on the bone quality. (AU)