Molecular bases of the control of cell proliferation and origin of neoplasms in the genomic and proteomic era

• Grant number: 01/10707-7
• Support Opportunities: Research Projects - Thematic Grants
• Start date: March 01, 2003
• End date: September 30, 2009
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Mari Cleide Sogayar
• Grantee: Mari Cleide Sogayar
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This project aims at updating and extending the Thematic project developed during the past 5 years (F APESP No. 95/9344-4). The General Objective is to reveal the molecular bases for normal/regulated processes of cell proliferation and differentiation and for the escape from this control that leads to tumor growth, using in vitro and in vivo transgenic systems (cell cultures and animals) in addition to cutting edge technologies of Genomics, Proteomics, Tissue Engineering and Bioinformatics. The following Specific Objectives are proposed: 1. expression analysis of genes associated with control of cell proliferation and differentiation and the origin of neoplasia; 2. searching, isolation and structural characterization of genes which are differentially expressed in the presence of mitogenic agents, differentiation-inducers, tumor virus and anti-tumor/anti-proliferative agents; 3. in vitro functional analysis of genes isolated in the previous item; 4. in vivo functional analysis of genes isolated in item 2, generating "knock-in" and "knock-out" transgenic animals; 5. isolation, culture, characterization and genetic modification of cells obtained from human and animal tissues and organs; 6. analysis and isolation of new genes from human and animal origin; 7. production of recombinant proteins of scientific and biotechnological interest; 8. generation of specific polyclonal antibodies to proteins of interest. The viability of this project is based on a consolidated Team of about 30 people, distributed in 2 well equipped laboratories (Cell and Molecular Biology Lab. and the Human Pancreatic lslet Unit) and on extensive networking with other Brazilian and foreign research groups. ln addition to generating new and relevant knowledge, we hope to continue to yield well-trained people who are qualified to use the knowledge generated in the project to develop technologies and to formulate new strategies for Cell and Molecular therapy of diseases. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA PAIVA, KATIUCIA BATISTA; ZAMBUZZI, WILLIAN FERNANDO; ACCORSI-MENDONCA, THAIS; TAGA, RUMIO; NUNES, FABIO DAUMAS; SOGAYAR, MARI CLEIDE; GRANJEIRO, JOSE MAURO. Rat forming incisor requires a rigorous ECM remodeling modulated by MMP/RECK balance. Journal of Molecular Histology, v. 40, n. 3, p. 201-207, JUN 2009. (07/00216-2, 99/10655-5, 07/04148-1, 01/10707-7)

CARDOSO DE OLIVEIRA DEMARCHI, ANA CLAUDIA; ZAMBUZZI, WILLIAN FERNANDO; SILVA PAIVA, KATIUCIA BATISTA; DA SILVA-VALENZUELA, MARIA DAS GRACAS; NUNES, FABIO DAUMAS; SAVIO FIGUEIRA, RITA DE CASSIA; SASAHARA, REGINA MAKI; ALMEIDA DEMASI, MARCOS ANGELO; BROCHADO WINNISCHOFER, SHEILA MARIA; SOGAYAR, MARI CLEIDE; et al. Development of secondary palate requires strict regulation of ECM remodeling: sequential distribution of RECK, MMP-2, MMP-3, and MMP-9. Cell and Tissue Research, v. 340, n. 1, p. 61-69, APR 2010. (01/10707-7)

TAKAMORI‚ E.R.; CRUZ‚ R.; GONÇALVEZ‚ F.; ZANETTI‚ R.V.; ZANETTI‚ A.; GRANJEIRO‚ J.M.. Effect of roughness of zirconia and titanium on fibroblast adhesion. ARTIFICIAL ORGANS, v. 32, n. 4, p. 305-309, 2008. (99/06460-4, 01/10707-7)

MENEZES‚ R.; BRAMANTE‚ C.M.; DA SILVA PAIVA‚ K.B.; LETRA‚ A.; CARNEIRO‚ E.; FERNANDO ZAMBUZZI‚ W.; GRANJEIRO‚ J.M.. Receptor activator NF [kappa] B-ligand and osteoprotegerin protein expression in human periapical cysts and granulomas. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, v. 102, n. 3, p. 404-409, 2006. (01/10707-7)

LOURO‚ R.; NAKAYA‚ H.; AMARAL‚ P.; FESTA‚ F.; SOGAYAR‚ M.; DA SILVA‚ A.; VERJOVSKI-ALMEIDA‚ S.; REIS‚ E.. Androgen responsive intronic non-coding RNAs. BMC Biology, v. 5, n. 1, p. 4, 2007. (01/10707-7, 02/13283-6)

FORNI, MARIA FERNANDA; MAIA LOBBA, ALINE RAMOS; PEREIRA FERREIRA, ALEXANDRE HAMILTON; SOGAYAR, MARI CLEIDE. Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin. PLoS One, v. 10, n. 10, OCT 13 2015. (01/10707-7)

TROMBETTA-LIMA, MARINA; BROCHADO WINNISCHOFER, SHEILA MARIA; ALMEIDA DEMASI, MARCOS ANGELO; ASTORINO FILHO, RENATO; OLIVEIRA CARREIRA, ANA CLAUDIA; WEI, BEIYANG; RIBAS, THAIS DE ASSIS; KONIG, MICHELLE SILBERSPITZ; BOWMAN-COLIN, CHRISTIAN; OBA-SHINJO, SUELI MIEKO; et al. Isolation and characterization of novel RECK tumor suppressor gene splice variants. ONCOTARGET, v. 6, n. 32, p. 33120-33133, OCT 20 2015. (04/12133-6, 01/10707-7)

FLÁVIO MONTEIRO-AMADO; IGOR IUCO CASTRO-SILVA; CRISTINA JARDELINO DE LIMA; FERNANDO AUGUSTO SOARES; LUIZ PAULO KOWALSKI; JOSÉ MAURO GRANJEIRO. Immunohistochemical evaluation of MMP-2, MMP-9 and CD31/microvascular density in squamous cell carcinomas of the floor of the mouth. Brazilian Dental Journal, v. 24, n. 1, p. 3-9, 2013-00-00. (01/10707-7)

THAIS ACCORSI-MENDONÇA; MÁRCIO BALTAZAR CONZ; TERESA CRISTINA BARROS; LÍDIA ÁGATA DE SENA; GLÓRIA DE ALMEIDA SOARES; JOSÉ MAURO GRANJEIRO. Physicochemical characterization of two deproteinized bovine xenografts. Brazilian Oral Research, v. 22, n. 1, p. 5-10, 2008-03-00. (01/10707-7, 99/10655-5)

ACCORSI-MENDONCA, THAIS; ZAMBUZZI, WILLIAN FERNANDO; BRAMANTE, CLOVIS MONTEIRO; CESTARI, TANIA MARI; TAGA, RUMIO; SADER, MARCIA; DE ALMEIDA SOARES, GLORIA DULCE; GRANJEIRO, JOSE MAURO. Biological monitoring of a xenomaterial for grafting: an evaluation in critical-size calvarial defects. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 22, n. 4, p. 997-1004, APR 2011. (01/10707-7, 99/10655-5)

ESTHER RIEKO TAKAMORI; EDUARDO ALEIXO FIGUEIRA; RUMIO TAGA; MARI CLEIDE SOGAYAR; JOSÉ MAURO GRANJEIRO. Evaluation of the cytocompatibility of mixed bovine bone. Brazilian Dental Journal, v. 18, n. 3, p. 179-184, 2007-00-00. (01/10707-7, 99/06460-4)

OLIVEIRA‚ R.C.; OLIVEIRA‚ F.H.G.; CESTARI‚ T.M.; TAGA‚ R.; GRANJEIRO‚ J.M.. Morphometric evaluation of the repair of critical-size defects using demineralized bovine bone and autogenous bone grafts in rat calvaria. Clinical Oral Implants Research, v. 19, n. 8, p. 749-754, 2008. (01/10707-7)