In vivo targetting of HIV- CD4+ t cell epitopes to dendritic cells

• Grant number: 14/15061-8
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2015
• End date: March 31, 2017
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Daniela Santoro Rosa
• Grantee: Daniela Santoro Rosa
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Edecio Cunha Neto ; Silvia Beatriz Boscardin

Abstract

The epidemic caused by the human immunodeficiency virus (HIV-1) is the most important emerging infection in the last decades. Despite advances in understanding the pathogenesis of the virus and the immune response against infection, there is no effective vaccine against HIV yet. Several lines of evidence indicate that neutralizing antibodies, CD4 + and CD8 + T lymphocytes play a major role in immunity against HIV. Vaccines that induce cellular response against HIV-1 developed so far, are mostly aimed at inducing CD8+ T lymphocyte responses. However, the role of specific CD4+ T responses in immunity against HIV-1, as evidenced by studies with infected patients and experimental vaccines have confirmed the importance of the development of a vaccine inducing these cell subtype. In previous studies, our group showed that immunization with a DNA vaccine in mice encoding multiple CD4+ T cells epitopes from HIV-1 was able to induce broad, polyfunctional and long-term CD4+ and CD8+ T cells specific responses. Although the results obtained with this vaccine formulation are quite promising, DNA vaccines have shown limited immunogenicity in humans. For this reason, in the recent years different platforms for vaccine production have been developed in order to transpose this barrier.An alternative that has been explored with success is based on in vivo targeting of antigens to dendritic cells (DCs). A sub-population of CD8+ DEC205+ DCs also express the DEC205 endocytic receptor. Several studies have shown that construction of an anti-DEC205 antibody fused to the antigen of interest is capable to induce strong antigen-specific humoral and cellular immune response. The aim of this project is to generate and produce a chimeric anti-DEC205 antibody encoding CD4+ epitopes of HIV and evaluate its immunogenicity in BALB / c mice. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NUNES, JETHE O. F.; APOSTOLICO, JULIANA DE SOUZA; ANDRADE, DAVID A. G.; RUIZ, FRANCIELI S.; FERNANDES, EDGAR R.; ANDERSEN, MONICA L.; KELLER, ALEXANDRE C.; ROSA, DANIELA S.. Sleep deprivation predisposes allergic mice to neutrophilic lung inflammation. Journal of Allergy and Clinical Immunology, v. 141, n. 3, p. 1018+, MAR 2018. (12/04692-1, 14/15061-8)

APOSTOLICO, JULIANA DE SOUZA; SANTOS LUNARDELLI, VICTORIA ALVES; YAMAMOTO, MARCIO MASSAO; SANTOS SOUZA, HIGO FERNANDO; CUNHA-NETO, EDECIO; BOSCARDIN, SILVIA BEATRIZ; ROSA, DANIELA SANTORO. Dendritic Cell Targeting Effectively Boosts T Cell Responses Elicited by an HIV Multiepitope DNA Vaccine. FRONTIERS IN IMMUNOLOGY, v. 8, FEB 7 2017. (14/15061-8, 13/11442-4)

APOSTOLICO, JULIANA DE SOUZA; SANTOS LUNARDELLI, VICTORIA ALVES; COIRADA, FERNANDA CAROLINE; BOSCARDIN, SILVIA BEATRIZ; ROSA, DANIELA SANTORO. Adjuvants: Classification, Modus Operandi, and Licensing. JOURNAL OF IMMUNOLOGY RESEARCH, v. 2016, p. 16-pg., 2016-01-01. (13/11442-4, 14/15061-8)

APOSTOLICO, JULIANA DE SOUZA; SANTOS LUNARDELLI, VICTORIA ALVES; COIRADA, FERNANDA CAROLINE; BOSCARDIN, SILVIA BEATRIZ; ROSA, DANIELA SANTORO. Adjuvants: Classification, Modus Operandi, and Licensing. CLINICAL & DEVELOPMENTAL IMMUNOLOGY, 2016. (14/15061-8, 13/11442-4)

PORCHIA, BRUNA F. M. M.; MORENO, ANA CAROLINA R.; RAMOS, RODRIGO N.; DINIZ, MARIANA O.; DE ANDRADE, LAIS HELENA T. M.; ROSA, DANIELA S.; BARBUTO, JOSE ALEXANDRE M.; BOSCARDIN, SILVIA B.; FERREIRA, LUIS CARLOS S.. Herpes Simplex Virus Glycoprotein D Targets a Specific Dendritic Cell Subset and Improves the Performance of Vaccines to Human Papillomavirus-Associated Tumors. MOLECULAR CANCER THERAPEUTICS, v. 16, n. 9, p. 1922-1933, SEP 1 2017. (09/54599-5, 16/00708-1, 11/20917-0, 15/16505-0, 11/08905-7, 11/51218-0, 14/15061-8)

ZANETI, ARTHUR BARUEL; YAMAMOTO, MARCIO MASSAO; SULCZEWSKI, FERNANDO BANDEIRA; ALMEIDA, BIANCA DA SILVA; SANTOS SOUZA, HIGO FERNANDO; FERREIRA, NATALIA SOARES; NASCIMENTO FABRIS MAEDA, DENICAR LINA; SALES, NATIELY SILVA; ROSA, DANIELA SANTORO; DE SOUZA FERREIRA, LUIS CARLOS; et al. Dendritic Cell Targeting Using a DNA Vaccine Induces Specific Antibodies and CD4(+) T Cells to the Dengue Virus Envelope Protein Domain III. FRONTIERS IN IMMUNOLOGY, v. 10, JAN 29 2019. (16/04477-4, 13/11442-4, 14/50631-0, 14/17595-0, 15/16565-2, 14/15061-8, 15/18874-2)