| Grant number: | 14/25494-9 |
| Support Opportunities: | Regular Research Grants |
| Start date: | March 01, 2015 |
| End date: | August 31, 2017 |
| Field of knowledge: | Biological Sciences - Biochemistry - Biochemistry of Microorganisms |
| Principal Investigator: | Maria Julia Manso Alves |
| Grantee: | Maria Julia Manso Alves |
| Host Institution: | Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
Abstract
Herein we propose to identify posttranslational modifications of proteins from T. cruzi in two distinct conditions, both relevants for the infection step: adhesion to extracellular matrix and exposure to mild pH decrease (pH 6). Levels of phosphorylation and S-nitrosylation of proteins will be determined in both conditions, with special attention to the flagellar proteins, since flagellum is a sensor element of the external medium and is lost in late amastigogenesis Proteins associated with the paraflagellar rod will be pull down by anti-PAR antibodies and identified. Identification of these proteins by MS/MS, as well as the possible involvement of NO/ NOS in this event will be pursued. Since S-nitrosylation (as well as others NO-dependent modifications) is related to the levels of NO, the activity of NO synthase and levels of cAMP and cGMP will be determined. The goal of the project is to understand the modifications at the molecular level induced in T. cruzi as a consequence of the interation with the extracellular matrix and lower pH, both essential conditions for the establishment of the parasite infection. (AU)
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