Identification of flagellar proteins from Trypanosoma cruzi modified due to the parasite adhesion to the host extracelullar matrix and cytoskeleton.

• Grant number: 13/16002-2
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): March 01, 2014
• Effective date (End): February 28, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Maria Julia Manso Alves
• Grantee: Eliciane Cevolani Mattos
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Chagas disease was firstly described in 1909 and since them, different aspects of this illness have being studied. The adhesion step of the infective forms (trypomastigotes) to components of the extracellular matrix (ECM) seems to be essential for the establishment of the parasite inside the host cells. Glycoproteins from the gp85/Trans-sialidase family are crucial in this step. Although it was also described the interaction of some members of this family with cytokeratins and vimentin from the host cell, its role in the infectivity of T. cruzi is unknown. It is also scarce the knowledge of the signaling events in the parasite as a consequence of the interactions described. Recently we described modifications in the phosphorylation level of proteins from T. cruzi, as paraflagellar rod protein and tubulin after incubation with laminin and fibronectin, components of ECM. These results pointed out the possible role of the flagellum from T. cruzi in the adhesion step. Since the importance of the flagellum as potential receptor and transducer of extracellular signs described in the literature by many laboratories, we decided to explore the flagellum proteome of T. cruzi submitted to different conditions. This project aims to compare the protein composition of the flagellum from trypomastigotes (infective form) and epimastigotes (non infective form) and the proteins from the flagellum modified during the adhesion of the parasite to the ECM and to the host cytokeratin 18, in order to understand the role of the flagellum in the host infection and development of the disease.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MATTOS, ELICIANE C.; CANUTO, GISELE; MANCHOLA, NUBIA C.; MAGALHAES, RUBENS D. M.; CROZIER, THOMAS W. M.; LAMONT, DOUGLAS J.; TAVARES, MARINA F. M.; COLLI, WALTER; FERGUSON, MICHAEL A. J.; ALVES, MARIA JULIA M.. Reprogramming of Trypanosoma cruzi metabolism triggered by parasite interaction with the host cell extracellular matrix. PLoS Neglected Tropical Diseases, v. 13, n. 2, FEB 2019. (16/14506-1, 14/25494-9, 18/03727-2, 13/16002-2)

MISERANI MAGALHAES, RUBENS DANIEL; MATTOS, ELICIANE CEVOLANI; ROZANSKI, ANDREI; FAVORETTO GALANTE, PEDRO ALEXANDRE; PALMISANO, GIUSEPPE; CRUZ, ANGELA KAYSEL; COLLI, WALTER; CAMARGO, ANAMARIA ARANHA; MANSO ALVES, MARIA JULIA. Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix. PLoS Neglected Tropical Diseases, v. 14, n. 5, MAY 2020. (14/10046-0, 13/16002-2, 18/15549-1, 18/18257-1, 14/25494-9, 14/06863-3)