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Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach

Grant number: 15/26818-5
Support type:Research Grants - Young Investigators Grants
Duration: October 01, 2016 - September 30, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Fabiana Fernandes Bressan
Grantee:Fabiana Fernandes Bressan
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Assoc. researchers:André Furugen Cesar de Andrade ; Carlos Eduardo Ambrósio ; Daniele dos Santos Martins ; Dimas Tadeu Covas ; Felipe Perecin ; Flávio Vieira Meirelles ; José Eduardo Krieger ; Lawrence Charles Smith ; Simone Kashima Haddad ; Virginia Picanço e Castro ; Wilson Araújo da Silva Junior
Associated scholarship(s):19/02811-2 - In vitro cellular reprogramming to pluripotency (IPS) in the swine model from non-transgenic and non-invasive methodology, BP.MS
19/03242-1 - Equine induced pluripotent stem cell (eiPSC) generation from episomal mechanism, BP.IC
18/24520-7 - Epigenetic modification mechanisms are activated by reactive oxygen (ro) in bovine induced pluripotent stem cells, BP.IC
+ associated scholarships 18/04009-6 - Effects of low oxygen tension in the reprogramming efficiency and manutention of induced pluripotent stem cells in equine, BP.DR
16/16841-2 - Trophectoderm complementation assay for IPS cells: a proposal for assessing pluripotency in cattle, BP.DR
17/02159-8 - Induction of cell pluripotency and in vitro differentiation in the porcine model as a translational model, BP.MS
17/02049-8 - Generation and cell culture of pluripotent cells in the pig model: the search for generation and differential characterization between primed and naïve cells, BP.IC
16/19580-5 - Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach, BP.JP - associated scholarships

Abstract

Cellular reprogramming technologies are a promising tool for the understanding of pluripotent cell biology and also to study several genetic and tissue-related disorders, enabling the investigation of the molecular events responsible for the acquisition of competency into pluri- and totipotency. This proposal aims to consolidate procedures for the generation of induced pluripotent stem (iPS) cells and germ cells in vitro from domestic species of commercial and biomedical interest (focus on cattle and swine) by investigating cellular and molecular mechanisms involved in cellular reprogramming, and by disseminating protocols and biological material for collaborations. Three general goals are proposed: 1) The standardization of safe and reproducible protocols of iPS generation, 2) The generation of germ cells, gametes and embryos from in vitro cultured cells, and 3) The investigation of transcriptional and epigenetic mechanisms of cellular reprogramming in domestic animals. The results achieved by this proposal will consolidate national research on nuclear and epigenetic reprogramming, allow the international insertion through scientific collaborations and high impact scientific publications, finally resulting in the formation of a multidisciplinary, competitive and collaborative research group both nationally and internationally. (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PESSOA, LAIS VICARI DE FIGUEIREDO; BRESSAN, FABIANA FERNANDES; FREUDE, KRISTINE KARLA. Induced pluripotent stem cells throughout the animal kingdom: Availability and applications. WORLD JOURNAL OF STEM CELLS, v. 11, n. 8, p. 491-505, AUG 26 2019. Web of Science Citations: 1.
SANGUINET, EDUARDO DE O.; SIQUEIRA, NATALY M.; MENEZES, FELIPE DE C.; RASIA, GISELE M.; LOTHHAMMER, NIVIA; SOARES, ROSANE M. D.; MEIRELLES, FLAVIO V.; BRESSAN, FABIANA F.; BOS-MIKICH, ADRIANA. Interaction of fibroblasts and induced pluripotent stem cells with poly(vinyl alcohol)-based hydrogel substrates. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, JUN 2019. Web of Science Citations: 0.
TRINDADE HILL, AMANDA BARACHO; BRESSAN, FABIANA FERNANDES; MURPHY, BRUCE D.; GARCIA, JOAQUIM MANSANO. Applications of mesenchymal stem cell technology in bovine species. STEM CELL RESEARCH & THERAPY, v. 10, JAN 24 2019. Web of Science Citations: 1.
DE FIGUEIREDO PESSOA, LAIS VICARI; LISBOA PIRES, PEDRO RATTO; DEL COLLADO, MAITE; GODOY PIERI, NAIRA CAROLINE; RECCHIA, KAIANA; SOUZA, ALINE FERNANDA; PERECIN, FELIPE; DA SILVEIRA, JULIANO COELHO; CESAR DE ANDRADE, ANDRE FURUGEN; AMBROSIO, CARLOS EDUARDO; BRESSAN, FABIANA FERNANDES; MEIRELLES, FLAVIO VIEIRA. Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues. STEM CELLS INTERNATIONAL, 2019. Web of Science Citations: 1.
DE CASTRO, RAQUEL V. G.; TAVARES, MARIANA R.; BRESSAN, FABIANA F.; PIERI, NAIRA C. G.; TRINDADE HILL, AMANDA BARACHO; SOUZA, ALINE F.; CRUZ, NATHAN DA R. N.; MARTINS, DANIELE S.; AMBROSIO, CARLOS E.; MEIRELLES, FLAVIO V.; GARCIA, JOAQUIM M. In vitro identification of a stem cell population from canine hair follicle bulge region. TISSUE & CELL, v. 50, p. 43-50, FEB 2018. Web of Science Citations: 1.
GODOY PIERI, NAIRA CAROLINE; DE SOUZA, ALINE FERNANDA; BOTIGELLI, RAMON CESAR; MACHADO, LUCAS SIMOES; AMBROSIO, CARLOS EDUARDO; MARTINS, DANIELE DOS SANTOS; CESAR DE ANDRADE, ANDRE FURUGEN; MEIRELLES, FLAVIO VIEIRA; HYTTEL, POUL; BRESSAN, FABIANA FERNANDES. Stem cells on regenerative and reproductive science in domestic animals. VETERINARY RESEARCH COMMUNICATIONS, v. 43, n. 1, p. 7-16, FEB 2018. Web of Science Citations: 2.
CURCIO, ALINNE GLORIA; BRESSAN, FABIANA FERNANDES; MEIRELLES, FLYVIO VIEIRA; BURLA DIAS, ANGELO JOSE. Achievements and perspectives in cloned and transgenic cattle production by nuclear transfer: influence of cell type, epigenetic status and new technology. ANIMAL REPRODUCTION, v. 14, n. 4, p. 1003-1013, OCT-DEC 2017. Web of Science Citations: 1.

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