Purification and immobilization of beta-glucosidase from Thermoascus aurantiacus using supermacroporous cryogels and application in bioprocesses

• Grant number: 17/16482-5
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Start date: October 01, 2017
• End date: July 31, 2020
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Roberto da Silva
• Grantee: Roberto da Silva
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Associated researchers: Paula Chequer Gouveia Mol

Abstract

Beta-glucosidases are enzymes that hydrolyze terminal beta-(1.4) glycoside bonds, essential function to many biological processes, being of great interest to biochemistry and biotechnology. This study involves the extraction and subsequent purification of ²-glucosidase of thermophilic fungus Thermoascus aurantiacus in a supermacroporous support of polyacrylamide produced in cryogenic conditions and chemically modified by the introduction of ionic groups N, N-dimethylaminoethyl methacrylamide. For this, different pH values and different flow rates of the mobile phase will be tested on the specific activity of the purified enzyme. After the purification step, an immobilization procedure will be studied using the same support. The effects of pH and temperature on the stability of free and immobilized enzymes will be determined, as well as the determination of the kinetic parameters of Michaelis-Menten model. Finally, the performance of the system containing the immobilized enzymes will be tested in the hydrolysis of isoflavone and degradation of pretreated sugar cane bagasse. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRASSATTO, PRISCILA APARECIDA CASCIATORI; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO; GOMES, ELENI; BOSCOLO, MAURICIO; DA SILVA, ROBERTO. Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse. SEMINA-CIENCIAS AGRARIAS, v. 41, n. 5, 1, p. 2097-2115, 2020. (13/01756-1, 18/00996-2, 17/16482-5, 11/07453-5, 10/12624-0, 11/21239-6)

DE CARVALHO TAVARES, IASNAIA MARIA; DE CASTILHOS, BONATTO MACHADO; MAURO, MARIA APARECIDA; RAMOS, AFONSO MOTA; DE SOUZA, REGINALDO TEODORO; GOMEZ-ALONSO, SERGIO; GOMES, ELENI; DA-SILVA, ROBERTO; HERMOSIN-GUTIERREZ, ISIDRO; LAGO-VANZELA, ELLEN SILVA. BRS Violeta (BRS Rubea x IAC 1398-21) grape juice powder produced by foam mat drying. Part I: Effect of drying temperature on phenolic compounds and antioxidant activity. Food Chemistry, v. 298, NOV 15 2019. (14/11514-8, 17/16482-5, 13/19057-2)

FRASSATTO, PRISCILA APARECIDA CASCIATORI; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO; GOMES, ELENI; BOSCOLO, MAURICIO; DA SILVA, ROBERTO. Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse. SEMINA-CIENCIAS AGRARIAS, v. 41, n. 5, p. 19-pg., 2020-01-01. (17/16482-5, 11/07453-5, 13/01756-1, 10/12624-0, 18/00996-2, 11/21239-6)

OLIVATI, CAROLINA; DE OLIVEIRA NISHIYAMA, YARA PAULA; DE SOUZA, REGINALDO TEODORO; JANZANTTI, NATALIA SOARES; MAURO, MARIA APARECIDA; GOMES, ELENI; HERMOSIN-GUTIERREZ, ISIDRO; DA SILVA, ROBERTO; LAGO-VANZELA, ELLEN SILVA. Effect of the pre-treatment and the drying process on the phenolic composition of raisins produced with a seedless Brazilian grape cultivar. Food Research International, v. 116, p. 190-199, FEB 2019. (14/11514-8, 17/16482-5)

DE CARVALHO TAVARES, IASNAIA MARIA; SUMERE, BEATRIZ ROCCHETTI; GOMEZ-ALONSO, SERGIO; GOMES, ELENI; HERMOSIN-GUTIERREZ, ISIDRO; DA-SILVA, ROBERTO; LAGO-VANZELA, ELLEN SILVA. Storage stability of the phenolic compounds, color and antioxidant activity of jambolan juice powder obtained by foam mat drying. Food Research International, v. 128, FEB 2020. (17/16482-5, 13/19057-2)

DE OLIVEIRA NASCIMENTO, CARLOS EDUARDO; DE OLIVEIRA SIMOES, LORENA CAIXETA; PEREIRA, JOSIANI DE CASSIA; DA SILVA, RONIVALDO RODRIGUES; DE LIMA, EVANDRO ANTONIO; DE ALMEIDA, GABRIEL CIMONETTI; PENNA, ANA LUCIA BARRETTO; BOSCOLO, MAURICIO; GOMES, ELENI; DA SILVA, ROBERTO. Application of a recombinant GH10 endoxylanase from Thermoascus aurantiacus for xylooligosaccharide production from sugarcane bagasse and probiotic bacterial growth. Journal of Biotechnology, v. 347, p. 8-pg., 2022-03-10. (17/16482-5)

COSCUETA, EZEQUIEL R.; BATISTA, PATRICIA; GOMES, JOSE ERICK GALINDO; DA SILVA, ROBERTO; PINTADO, MARIA MANUELA. Screening of Novel Bioactive Peptides from Goat Casein: In Silico to In Vitro Validation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 5, p. 9-pg., 2022-03-01. (17/16482-5)

DE CARVALHO TAVARES, IASNAIA MARIA; UMSZA-GUEZ, MARCELO ANDRES; MARTIN, NATALIA; TOBAL, THAISE MARIA; BOSCOLO, MAURICIO; GOMES, ELENI; DA-SILVA, ROBERTO; LAGO-VANZELA, ELLEN SILVA. The improvement of grape juice quality using Thermomucor Indicae-Seudaticae pectinase. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, v. 57, n. 4, NOV 2019. (13/19057-2, 10/09998-6, 17/16482-5)

CUTRIM FARIAS, FLAVIA DE AQUINO; DE SOUZA MORETTI, MARCIA MARIA; COSTA, MARIANA SOUZA; BORDIGNONJUNIOR, SIDNEI EMILIO; CAVALCANTE, KIANYSIRLEY BRANDAO; BOSCOLO, MAURICIO; GOMES, ELENI; LANDI FRANCO, CELIA MARIA; DA SILVA, ROBERTO. Structural and physicochemical characteristics of taioba starch in comparison with cassava starch and its potential for ethanol production. INDUSTRIAL CROPS AND PRODUCTS, v. 157, DEC 1 2020. (17/16482-5)

GOUVEIA MOL, PAULA CHEQUER; ALCANTARA VERISSIMO, LIZZY AYRA; MINIM, LUIS ANTONIO; BOSCOLO, MAURICIO; GOMES, ELENI; DA SILVA, ROBERTO. Production and capture of beta-glucosidase from Thermoascus aurantiacus using a tailor made anionic cryogel. Process Biochemistry, v. 82, p. 75-83, JUL 2019. (17/16482-5, 16/17812-6)

DE OLIVEIRA SIMOES, LORENA CAIXETA; DA SILVA, RONIVALDO RODRIGUES; DE OLIVEIRA NASCIMENTO, CARLOS EDUARDO; BOSCOLO, MAURICIO; GOMES, ELENI; DA SILVA, ROBERTO. Purification and Physicochemical Characterization of a Novel Thermostable Xylanase Secreted by the Fungus Myceliophthora heterothallica F.2.1.4. Applied Biochemistry and Biotechnology, v. 188, n. 4, p. 991-1008, AUG 2019. (17/16482-5)

GALINDO GOMES, JOSE ERICK; EVARISTO DA SILVA NASCIMENTO, TALITA CAMILA; DE SOUZA-MOTTA, CRISTINA MARIA; AGAMEZ MONTALVO, GUALBERTO SEGUNDO; BOSCOLO, MAURICIO; GOMES, ELENI; MOREIRA, KEILA APARECIDA; PINTADO, MARIA MANUELA; DA SILVA, ROBERTO. Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates. OURNAL OF FOOD MEASUREMENT AND CHARACTERIZATIO, v. 16, n. 6, p. 15-pg., 2022-08-12. (17/16482-5, 14/13700-3)

GOUVEIA MOL, PAULA CHEQUER; ALCANTARA VERISSIMO, LIZZY AYRA; MINIM, LUIS ANTONIO; DA SILVA, ROBERTO. Adsorption and immobilization of beta-glucosidase from Thermoascus aurantiacus on macroporous cryogel by hydrophobic interaction. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. N/A, p. 11-pg., 2022-06-03. (16/17812-6, 17/16482-5)

MOL, PAULA CHEQUER GOUVEIA; QUILLES, JOSE CARLOS; VERISSIMO, LIZZY AYRA ALCANTARA; BOSCOLO, MAURICIO; GOMES, ELENI; MINIM, LUIS ANTONIO; DA SILVA, ROBERTO. B-glucosidase: An overview on immobilization and some aspects of structure, function, applications and cost. Process Biochemistry, v. 130, p. 14-pg., 2023-04-05. (17/16482-5, 16/17812-6)