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Purification and immobilization of beta-glucosidase from Thermoascus aurantiacus using supermacroporous cryogels and application in bioprocesses

Abstract

Beta-glucosidases are enzymes that hydrolyze terminal beta-(1.4) glycoside bonds, essential function to many biological processes, being of great interest to biochemistry and biotechnology. This study involves the extraction and subsequent purification of ²-glucosidase of thermophilic fungus Thermoascus aurantiacus in a supermacroporous support of polyacrylamide produced in cryogenic conditions and chemically modified by the introduction of ionic groups N, N-dimethylaminoethyl methacrylamide. For this, different pH values and different flow rates of the mobile phase will be tested on the specific activity of the purified enzyme. After the purification step, an immobilization procedure will be studied using the same support. The effects of pH and temperature on the stability of free and immobilized enzymes will be determined, as well as the determination of the kinetic parameters of Michaelis-Menten model. Finally, the performance of the system containing the immobilized enzymes will be tested in the hydrolysis of isoflavone and degradation of pretreated sugar cane bagasse. (AU)

Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CUTRIM FARIAS, FLAVIA DE AQUINO; DE SOUZA MORETTI, MARCIA MARIA; COSTA, MARIANA SOUZA; BORDIGNONJUNIOR, SIDNEI EMILIO; CAVALCANTE, KIANYSIRLEY BRANDAO; BOSCOLO, MAURICIO; GOMES, ELENI; LANDI FRANCO, CELIA MARIA; DA SILVA, ROBERTO. Structural and physicochemical characteristics of taioba starch in comparison with cassava starch and its potential for ethanol production. INDUSTRIAL CROPS AND PRODUCTS, v. 157, DEC 1 2020. Web of Science Citations: 0.
DE CARVALHO TAVARES, IASNAIA MARIA; SUMERE, BEATRIZ ROCCHETTI; GOMEZ-ALONSO, SERGIO; GOMES, ELENI; HERMOSIN-GUTIERREZ, ISIDRO; DA-SILVA, ROBERTO; LAGO-VANZELA, ELLEN SILVA. Storage stability of the phenolic compounds, color and antioxidant activity of jambolan juice powder obtained by foam mat drying. Food Research International, v. 128, FEB 2020. Web of Science Citations: 0.
FRASSATTO, PRISCILA APARECIDA CASCIATORI; CASCIATORI, FERNANDA PERPETUA; THOMEO, JOAO CLAUDIO; GOMES, ELENI; BOSCOLO, MAURICIO; DA SILVA, ROBERTO. Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse. SEMINA-CIENCIAS AGRARIAS, v. 41, n. 5, 1, p. 2097-2115, 2020. Web of Science Citations: 1.
DE CARVALHO TAVARES, IASNAIA MARIA; DE CASTILHOS, BONATTO MACHADO; MAURO, MARIA APARECIDA; RAMOS, AFONSO MOTA; DE SOUZA, REGINALDO TEODORO; GOMEZ-ALONSO, SERGIO; GOMES, ELENI; DA-SILVA, ROBERTO; HERMOSIN-GUTIERREZ, ISIDRO; LAGO-VANZELA, ELLEN SILVA. BRS Violeta (BRS Rubea x IAC 1398-21) grape juice powder produced by foam mat drying. Part I: Effect of drying temperature on phenolic compounds and antioxidant activity. Food Chemistry, v. 298, NOV 15 2019. Web of Science Citations: 1.
DE CARVALHO TAVARES, IASNAIA MARIA; UMSZA-GUEZ, MARCELO ANDRES; MARTIN, NATALIA; TOBAL, THAISE MARIA; BOSCOLO, MAURICIO; GOMES, ELENI; DA-SILVA, ROBERTO; LAGO-VANZELA, ELLEN SILVA. The improvement of grape juice quality using Thermomucor Indicae-Seudaticae pectinase. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, v. 57, n. 4 NOV 2019. Web of Science Citations: 0.
DE OLIVEIRA SIMOES, LORENA CAIXETA; DA SILVA, RONIVALDO RODRIGUES; DE OLIVEIRA NASCIMENTO, CARLOS EDUARDO; BOSCOLO, MAURICIO; GOMES, ELENI; DA SILVA, ROBERTO. Purification and Physicochemical Characterization of a Novel Thermostable Xylanase Secreted by the Fungus Myceliophthora heterothallica F.2.1.4. Applied Biochemistry and Biotechnology, v. 188, n. 4, p. 991-1008, AUG 2019. Web of Science Citations: 3.
GOUVEIA MOL, PAULA CHEQUER; ALCANTARA VERISSIMO, LIZZY AYRA; MINIM, LUIS ANTONIO; BOSCOLO, MAURICIO; GOMES, ELENI; DA SILVA, ROBERTO. Production and capture of beta-glucosidase from Thermoascus aurantiacus using a tailor made anionic cryogel. Process Biochemistry, v. 82, p. 75-83, JUL 2019. Web of Science Citations: 1.
OLIVATI, CAROLINA; DE OLIVEIRA NISHIYAMA, YARA PAULA; DE SOUZA, REGINALDO TEODORO; JANZANTTI, NATALIA SOARES; MAURO, MARIA APARECIDA; GOMES, ELENI; HERMOSIN-GUTIERREZ, ISIDRO; DA SILVA, ROBERTO; LAGO-VANZELA, ELLEN SILVA. Effect of the pre-treatment and the drying process on the phenolic composition of raisins produced with a seedless Brazilian grape cultivar. Food Research International, v. 116, p. 190-199, FEB 2019. Web of Science Citations: 0.

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