Effect of probiotic fermented milk in cellular immune response of Balb/c mice colon

• Grant number: 08/10167-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2009
• End date: October 31, 2012
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Technology
• Principal Investigator: Marice Nogueira de Oliveira
• Grantee: Cristina Stewart Bittencourt Bogsan
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The functional food industries are in expansion mainly by probiotic and prebiotic products. Studies have shown some probiotic strains develop immune modulation effect, however, these results are controversial and the mechanisms are not been understood. Although, some probiotic strains increase IL-10 and g-INF release modulating the immune response, this response is weaker in probiotic strains than in pathogenic gram-positive bacteria. The major aim of the present project is study the effect of probiotic fermented milk in cellular immune response of Balb/c mice colon. The specific objectives are: (i) to determine the immunomodulation of the milk added of probiotic in normal mice; (ii) to identify the cellular types implied in immune specific response and, (iii) to co-localize them in histological sections. Dairy products will be prepared in which variable form will be the formularizations (a) milk; (b) fermented milk and (c) fermented and pasteurized milk, all using Bifidobacterium subsp. lactis HOWARU HN019 strain. The skimmed milk will be used as control. The immune effect will be evaluated by histological sections and the linfocitic infiltrated will be analyzed by flow citometry and confocal microscopy.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOGSAN, C. S. B.; FERREIRA, L.; MALDONADO, C.; PERDIGON, G.; ALMEIDA, S. R.; OLIVEIRA, M. N.. Fermented or unfermented milk using Bifidobacterium animalis subsp lactis HN019: Technological approach determines the probiotic modulation of mucosal cellular immunity. Food Research International, v. 64, p. 283-288, OCT 2014. (08/10167-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BOGSAN, Cristina Stewart Bittencourt. Effect of probiotic fermented milk in immune cellular response of BALB/c mice colon. 2012. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.