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Use microsatellite markers relatedness to estimate within open-pollinated families of the dioecious tree species: a case study of Myracrodruon urundeuva

Grant number: 09/12594-7
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2010
Effective date (End): February 29, 2012
Field of knowledge:Agronomical Sciences - Forestry Resources and Forestry Engineering
Principal Investigator:Alexandre Magno Sebbenn
Grantee:Marcela Aparecida de Moraes
Home Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil


Know the relatedness within open-pollinated families is a long time interest due the implications which this relatedness have on the estimative of the addictive genetic variation, effective population size and determination of the number of seed-tree for seed collect aiming ex situ conservation, tree breeding and environmental restoration. Thus, the aim of this project is to estimate and to compare different methods of to compute the coancestry coefficient within open-pollinated families of the dioecious tropical tree species, Myracrodruon urundeuva using microsatellite loci. Three methods of estimation of coancestry coefficient were compared: i) the estimative of the coancestry coefficient within families from mating system parameters, according with Ritland (1989) method; ii) the estimative of the coancestry coefficient within families from the measured of pollen gene poll differentiation among different seed-trees, with base in TWOGENER analysis; iii) the estimative of the coancestry coefficient within families from Loisselle et al. (1995) estimator and using the method proposed by Hardy et al. (2004). The last method only distings half-sibs and full-sibs within families and, thus, is adequate only for diocious species. Thus, open-pollinated families from seven populations of M. urundeuva, genotyped for eight microsatellite loci, will be used for this work. This study will contribute for breeding and conservation programs by proposition of the coefficient of relatedness to be used for to estimate the addictive genetic variation in M. urundeuva populations, as well as for to estimate the effective population size within families and to determine the number of seed-tree necessary for seed collect aiming ex situ conservations and environmental restoration.

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MORAES, Marcela Aparecida de. Uso de marcadores microssatélites para estimar parentescos dentro de progênies de polinização aberta de espécies arbóreas dióicas: um estudo de caso de Myracrodruon urundeuva (F.F. & M.F. Allemão). 2012. 80 f. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Engenharia (Campus de Ilha Solteira). Ilha Solteira.

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