Production of second generation ethanol from pentoses by Pichia stipitis using extractive fermentation process vacuum

• Grant number: 10/01606-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2010
• End date: April 30, 2014
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Francisco Maugeri Filho
• Grantee: Daniele Farias
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The biotechnological production of second generation ethanol through the cultivation of Pichia stipidis in hemicellulose hydrolyzates obtained from agro-industrial waste is of great economic interest. This is because, this process can add value to this waste, replace fossil fuels, and promote a complete recovery of materials. Adding to this, the use of fermentation media with high concentration of substrate is of great interest to industry because it reduces significantly the volume of vats and vinasse. Large amounts of ethanol in the middle consume less energy in the extraction process. Large amounts of ethanol in the culture medium consume less energy in the extraction process. However, this high concentration of ethanol inhibits the process, emerging from the same need to be removed from the environment once it is produced. The use of extraction techniques improves the performance of the process. The use of the flash evaporator allows to use high concentrations of sugars, which results in increased production of ethanol, reducing the cost of destillation. Facing this, the objective of this study is to develop the production of second generation ethanol in a continuous fermentation process coupled to a vacuum evaporator type flash, from carbon source concentrated from pentoses derived from enzymatically hydrolyzed bagasse enriched by mixing with sugar cane molasses in the process using high cell concentration of P. stipitis. The reason for the employment of this technology is due to the low tolerance of the microorganism to ethanol and low productivity. It is hoped that this system solve these problems, because this process allows to operate with low concentration of ethanol, even when working with highly concentrated must and high concentrations of microorganism, which allows higher conversion rates. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FARIAS, Daniele. Production of second generation ethanol by Schefferspmyces stipitis from pentoses by vacuum extractive process. 2014. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.