Large-scale study of transcriptional alterations associated with the development of metastasis in oral cancer.

• Grant number: 11/13315-4
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2011
• Effective date (End): October 01, 2015
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal researcher: Emmanuel Dias-Neto
• Grantee: Frederico Omar Gleber Netto
• Home Institution: A C Camargo Cancer Center. Fundação Antonio Prudente (FAP). São Paulo , SP, Brazil
• Associated research grant: 10/51168-0 - Environmental, clinical, histopathological and molecular factors associated with development and prognosis of head and neck squamous cell carcinomas, AP.TEM
• Associated scholarship(s): 13/09142-2 - Study of salivary markers of nodal metastasis in oral squamous cell carcinoma, BE.EP.DR

Abstract

Squamous cell carcinomas of the mouth (SCCM) are aggressive neoplasms that develop from the keratinocytes lining the oral mucosa and represent over 90% of tumors that occur in the mouth. Brazil has the highest incidence of SCCM among developing countries, and one of the world's highest occurrence rates. The main prognostic factor for poor survival in SCCM is the occurrence of metastatic disease in regional lymph nodes. Thus, the ability to predict this outcome will help identify patients who may benefit from a more radical surgical approach, while preserving those presenting less aggressive tumors, potentially contributing to greater and better survival rates. In this project we will investigate whether gene expression signatures determined from large-scale sequencing of cDNAs (RNASeq), can predict tumor lymph node invasion capabilities. For this, the complete transcriptome of small tumors (T1/T2) presenting lymph node disease will be compared with larger tumors (T3), with negative lymph nodes, derived from the same region. The large-scale sequencing will be done on the ABI5500 platform and computational analysis will be used to identify transcripts, gene-fusions or splicing isoforms as well as metabolic pathways associated with lymph node invasion potential. The findings will be assessed in independent samples, in order to challenge the validity of the potential markers set forth herein, hoping to determine clinically valuable biomarkers.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GLEBER-NETTO, FREDERICO O.; NESKEY, DAVID; DE MATTOS COSTA, ANA FLAVIA; KATARIA, PRANAV; RAO, XIAYU; WANG, JING; KOWALSKI, LUIZ PAULO; PICKERING, CURTIS R.; DIAS-NETO, EMMANUEL; MYERS, JEFFREY N. Functionally impactfulTP53mutations are associated with increased risk of extranodal extension in clinically advanced oral squamous cell carcinoma. Cancer, AUG 2020. Web of Science Citations: 0.

GLEBER-NETTO, FREDERICO OMAR; YAKOB, MAHA; LI, FENG; FENG, ZIDING; DAI, JIANLIANG; KAO, HUANG-KAI; CHANG, YU-LIANG; CHANG, KAI-PING; WONG, DAVID T. W. Salivary Biomarkers for Detection of Oral Squamous Cell Carcinoma in a Taiwanese Population. Clinical Cancer Research, v. 22, n. 13, p. 3340-3347, JUL 1 2016. Web of Science Citations: 12.

GLEBER-NETTO, FREDERICO O.; BRAAKHUIS, BOUDEWIJN J. M.; TRIANTAFYLLOU, ASTERIOS; TAKES, ROBERT P.; KELNER, NATALIE; RODRIGO, JUAN P.; STROJAN, PRIMOZ; VANDER POORTEN, VINCENT; RAPIDIS, ALEXANDER D.; RINALDO, ALESSANDRA; BRAKENHOFF, RUUD H.; FERLITO, ALFIO; KOWALSKI, LUIZ P. Molecular events in relapsed oral squamous cell carcinoma: Recurrence vs secondary primary tumor. Oral Oncology, v. 51, n. 8, p. 738-744, AUG 2015. Web of Science Citations: 14.