DNA extraction methods for the study of rumen microbial population in cattle fed w...
| Grant number: | 12/12779-0 |
| Support type: | Scholarships in Brazil - Master |
| Effective date (Start): | October 01, 2012 |
| Effective date (End): | February 28, 2014 |
| Field of knowledge: | Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding |
| Principal Investigator: | Jane Maria Bertocco Ezequiel |
| Grantee: | Marco Túlio Costa Almeida |
| Home Institution: | Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil |
Abstract This study will evaluate the association of glycerin, the biodiesel production residue, to additives essential oils (Essential - Oligobasics ®) and monensina (Rumensin - Elanco ®) in the diet of beef cattle, on nutrient intake, parameters ruminal (pH and ammonia nitrogen), production and quality of mass microbial, production gases methane and carbon dioxide, and in vitro digestibility of the diets.Five diets isoproteic (12.1% crude protein in dry matter) and isoenergetic (2.6 Mcal / kg metabolizable energy of dry matter), ratio roughage:concentrate of 30:70 and denominated control C (without glycerin and additives), EO (essential oils without glycerin); MON (monensin without glycerin); OEG (essential oils with glycerin), and MONG (monensin with glycerin). The introduction of glycerin in the treatment OEG and MONG will be 50% the dry matter in the feed total, and the inclusions of sodium monensin and essential oils will be 0.3 g / kg and 0.5 g / kg of dry matter, respectively. For determination of intake, ruminal parameters, gas production and microbial mass will be used five Nellore with approximately 400 kg body weight, with permanent cannulas in the rumen, in Latin square experimental design (5 x 5) in a factorial 2 x 2 + 1 (monensin or essential oils × absence or presence of glycerin + control). As a tool to aid the statistical analysis will be utilized the SAS (1993). | |