Cloning of a Brazilian isolate of chrysanthemum stunt viroid and development of molecular diagnostic technique

• Grant number: 12/20570-3
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: January 07, 2013
• End date: March 06, 2013
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Marcelo Eiras
• Grantee: Danielle Gobatto
• Supervisor: José Antonio Daròs
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Institution abroad: Consejo Superior de Investigaciones Científicas (CSIC), Spain
• Associated to the scholarship: 11/02721-1 - Viroids in chrysanthemum: survey, identification, characterization, source of resistance evaluation and development of diagnostic methods, BP.MS

Abstract

In Brazil, the ornamental species of flowers and plants market moves, annually, more than three billion dollars. The chrysanthemum (Dendranthema spp., Asteraceae) is considered one of the most valuable commercial ornamental, being produced in more than 100 municipalities in the State of São Paulo. Diseases induced by virus and viroids have been a serious problem for this crop in several countries, mainly by vegetative propagation, which facilitates the spread of theses pathogens. Viroids can cause serious damages to the culture, specially Chrysanthemum stunt viroid (CSVd, genus Pospiviroid, family Pospiviroidae), considered an EPPO A2 quarantine pest, can induce dwarf and retard flowering. In Brazil, there are few studies on these pathogens, with a single report, in 1990, of a possible occurrence of CSVd in chrysanthemum in the State of São Paulo. This project aims to: (i) identify and purify a Brazilian isolate of CSVd collected in Atibaia, São Paulo; (ii) clone a monomer of CSVd; and (iii) synthesize DNA and RNA probes (radioactive and non-radioactive) of negative polarity for CSVd detection by Northern- and dot-blot hybridization. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOBATTO, D.; CHAVES, A. L. R.; HARAKAVA, R.; MARQUE, J. M.; DAROS, J. A.; EIRAS, M.. CHRYSANTHEMUM STUNT VIROID IN BRAZIL: SURVEY, IDENTIFICATION, BIOLOGICAL AND MOLECULAR CHARACTERIZATION AND DETECTION METHODS. JOURNAL OF PLANT PATHOLOGY, v. 96, n. 1, p. 111-119, 2014. (11/02721-1, 12/20570-3)