Cerebral perfusion changes in obese subjects after administration of 75 grams of glucose: SPECT study, controlled, blinded and randomized

• Grant number: 12/21579-4
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2013
• End date: March 31, 2015
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Julio Sergio Marchini
• Grantee: José Henrique da Silva
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The obesity pathophysiology involves a complex interplay between peripheral flags and the brain metabolism. This study has the hypothesis that the stimuli elicited by ingested nutrients results in cerebral perfusion alterations related to adipose tissue excess. Objective: Measure brain perfusion changes with SPECT (Single-Photon Emission Tomography) after stimulation with 75 grams of glucose and avaluate them by Statistical Parametric Mapping® (SPM) and SISCOM (ANALYZE 10.0®) softwares. Also will be determinate the serum levels of glucose, insulin, leptin, the basal metabolic rate by indirect calorimetry (IC) and body composition by bioelectrical impedance analysis (BIA). Finally, the cerebral perfusion changes and other data will be correlated to the obesity pathophysiology. Methods: A blind, randomized, controlled, and two groups of people, being the experiment subject control of yourself. One "O" group with ten obese (18 to 40 years, BMI between 30 and 40 kg/m2) and a "NO" group with ten non-obese volunteers (same age group, BMI between 18.5 and 24.9 kg/m2, living in similar conditions to the obese), with no morbidities. The subjects of both groups will be submitted to nutritional status assessment, IC, BIA and Brain Magnetic Resonance, being this last one for the analysis of the SPECTs by SISCOM. Subsequently all the patients will be randomized into subgroups. "O-1" and "NO-1" (cases) receiving an oral solution with 75 grams of glucose after 12 hours of fasting and "O-2" and "NO-2" (controls) receiving the same volume of water under the same conditions. All assessed with SPECT and serum glucose, insulin and leptin before and after stimulation. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVAH, JOSE HENRIQUE; MARCHINI, JULIO SERGIO; MARTIRES LIMA, CRISTIANE MARIA; NICOLETTI, CAROLINA FERREIRA; SANTOS, LEONARDO ALEXANDRE; ITIKAWA, EMERSON NOBUYUKI; TREVISAN, ANA CAROLINA; PITELLA, FELIPE ARRIVA; KATO, MERY; IUCIF JUNIOR, NELSON; et al. Regional cerebral blood flow at rest in obesity. NUTRITION, v. 79-80, NOV-DEC 2020. (12/21579-4, 10/19821-6, 12/03485-2, 12/21626-2)

LARA CASSANI, ROBERTA SOARES; FASSINI, PRISCILA GIACOMO; SILVAH, JOSE HENRIQUE; MARTIRES LIMA, CRISTIANE MARIA; MARCHINI, JULIO SERGIO. RETRACTED: Impact of weight loss diet associated with flaxseed on inflammatory markers in men with cardiovascular risk factors: a clinical study (Retracted article. See vol. 15, pg. 59, 2016). NUTRITION JOURNAL, v. 14, JAN 10 2015. (12/21579-4, 12/21626-2, 12/22543-3)

SILVAH, JOSE HENRIQUE; MARTIRES DE LIMA, CRISTIANE MARIA; DEL LAMA DE UNAMUNO, MARIA DO ROSARIO; ALVES SCHETINO, MARCO ANTONIO; LEITE SCHETINO, LUANA PEREIRA D; FASSINI, PRISCILA GIACOMO; CUNHA MORAES BRANDAO, CAMILA FERNANDA COSTA E; BASILE-FILHO, ANIBAL; CARVALHO DA CUNHA, SELMA FREIRE; MARCHINI, JULIO SERGIO. Body surface infrared thermometry in patients with central venous cateter-related infections. Einstein (São Paulo), v. 13, n. 3, p. 364-369, JUL-SEP 2015. (12/21579-4, 12/21626-2, 12/22543-3)

SILVAH, JOSE HENRIQUE; MARTIRES DE LIMA, CRISTIANE MARIA; DEL LAMA DE UNAMUNO, MARIA DO ROSARIO; ALVES SCHETINO, MARCO ANTONIO; LEITE SCHETINO, LUANA PEREIRA D; FASSINI, PRISCILA GIACOMO; CUNHA MORAES BRANDAO, CAMILA FERNANDA COSTA E; BASILE-FILHO, ANIBAL; CARVALHO DA CUNHA, SELMA FREIRE; MARCHINI, JULIO SERGIO. Body surface infrared thermometry in patients with central venous cateter-related infections. Einstein (São Paulo), v. 13, n. 3, p. 6-pg., 2015-07-01. (12/21626-2, 12/21579-4, 12/22543-3)

LARA CASSANI, ROBERTA SOARES; FASSINI, PRISCILA GIACOMO; SILVAH, JOSE HENRIQUE; MARTIRES LIMA, CRISTIANE MARIA; MARCHINI, JULIO SERGIO. Impact of weight loss diet associated with flaxseed on inflammatory markers in men with cardiovascular risk factors: a clinical study (Retracted article. See vol. 15, pg. 59, 2016). NUTRITION JOURNAL, v. 14, p. 8-pg., 2015-01-10. (12/22543-3, 12/21579-4, 12/21626-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, José Henrique da. Cerebral perfusion changes in obese subjects after administration of 75 grams of glucose. A SPECT, controlled, blinded and randomized study. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.