Genomic, proteomic and metabolomic study of gastrointestinal tissue for patients with type 2 Diabetes mellitus after Roux-en-Y gastric bypass

• Grant number: 13/03246-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: April 01, 2013
• End date: March 31, 2017
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Dan Linetzky Waitzberg
• Grantee: Priscila Sala Kobal
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 11/09612-3 - Changes in gene expression in gastric and intestinal tissue of type 2 Diabetes patients submitted to Roux-en-Y gastric bypass, AP.TEM
• Associated scholarship(s): 14/07742-5 - Genomic, proteomic and metabolomic study of gastrointestinal tissue for patients with type 2 diabetes mellitus after Roux-en-Y gastric bypass, BE.EP.DD

Abstract

Type 2 diabetes mellitus (T2DM) is a relevant public health and obesity has been associated as the major risk factor. Roux-en-Y gastric bypass is an effective method to treat severe obesity, and its metabolic benefits lead to early and complete remission of T2DM, even before a considerable weight loss. Experimentally, the anatomical changes induced by RYGB can change genes expression in gastrointestinal tissue related to glucose control determinants to T2DM reversal. However, there aren't clinical studies that prove these findings in humans. Some gastrointestinal hormones already have been studied as possible mediators of glycemic control improvement after metabolic surgery, such as, ghrelin, peptide YY, enteroglucagon and incretins (GLP-1 and GIP). Due to endocrine effects of Roux-en-Y gastric bypass, it is reasonable to believe that other hormones and mechanisms are involved in glycemic homeostasis after surgery. The genomic, proteomic and metabolomics studies of gastrointestinal tissue in T2DM patients after Roux-en-Y gastric bypass can provide a better understanding of the T2DM pathophysiology enabling the study of new therapeutic targets for the treatment of this clinical condition. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FONSECA, DANIELLE CRISTINA; SALA, PRISCILA; SINGER, JOELLE; SINGER, PIERRE; TORRINHAS, RAQUEL SUSANA; WAITZBERG, DAN LINETZKY. Upregulation of Ghrelin Gene Expression in the Excluded Stomach of Obese Women with Type 2 Diabetes After Roux-en-Y Gastric Bypass in the SURMetaGIT Study. OBESITY SURGERY, v. 28, n. 3, p. 877-880, MAR 2018. (13/03246-0, 11/09612-3)

SALA, PRISCILA; BELARMINO, GILIANE; MACHADO, NATASHA MENDONCA; CARDINELLI, CAMILA SIQUEIRA; AL ASSAL, KARINA; SILVA, MARIANE MARQUES; FONSECA, DANIELLE CRISTINA; ISHIDA, ROBSON KIYOSHI; SANTO, MARCO AURELIO; HOURNEAUX DE MOURA, EDUARDO GUIMARAES; et al. The SURMetaGIT study: Design and rationale for a prospective pan-omics examination of the gastrointestinal response to Roux-en-Y gastric bypass surgery. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, v. 44, n. 6, p. 1359-1375, DEC 2016. (15/22820-5, 11/09612-3, 14/05220-1, 13/03246-0, 14/07742-5, 12/23762-0, 16/01259-6, 13/23355-9)

SALA, PRISCILA CAMPOS; TORRINHAS, RAQUEL SUSANA; GIANNELLA-NETO, DANIEL; WAITZBERG, DAN LINETZKY. Relationship between gut hormones and glucose homeostasis after bariatric surgery. DIABETOLOGY & METABOLIC SYNDROME, v. 6, AUG 16 2014. (13/03246-0, 11/09612-3)

SALA, PRISCILA; TORRINHAS, RAQUEL SUSANA; FONSECA, DANIELLE CRISTINA; HEYMSFIELD, STEVEN; GIANNELLA-NETO, DANIEL; WAITZBERG, DAN LINETZKY. Type 2 Diabetes Remission After Roux-en-Y Gastric Bypass: Evidence for Increased Expression of Jejunal Genes Encoding Regenerating Pancreatic Islet-Derived Proteins as a Potential Mechanism. OBESITY SURGERY, v. 27, n. 4, p. 1123-1127, APR 2017. (13/03246-0, 11/09612-3)

SALA, PRISCILA; BELARMINO, GILIANE; TORRINHAS, RAQUEL S.; MACHADO, NATASHA M.; FONSECA, DANIELLE C.; RAVACCI, GRAZIELA R.; ISHIDA, ROBSON K.; GUARDA, ISMAEL F. M. S.; DE MOURA, EDUARDO G.; SAKAI, PAULO; et al. Gastrointestinal Transcriptomic Response of Metabolic Vitamin B12 Pathways in Roux-en-Y Gastric Bypass. CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY, v. 8, JAN 2017. (13/03246-0, 11/09612-3)

SALA, PRISCILA; MATOS DE MIRANDA TORRINHAS, RAQUEL SUSANA; FONSECA, DANIELLE CRISTINA; RAVACCI, GRAZIELA ROSA; WAITZBERG, DAN LINETZKY; GIANNELLA-NETO, DANIEL. Tissue-specific methylation profile in obese patients with type 2 diabetes before and after Roux-en-Y gastric bypass. DIABETOLOGY & METABOLIC SYNDROME, v. 9, FEB 22 2017. (13/03246-0, 11/09612-3)

SALA, PRISCILA; MACHADO, NATASHA MENDONCA; TORRINHAS, RAQUEL SUSANA MATOS DE MIRANDA; FONSECA, DANIELLE C.; FERREIRA, BEATRIZ A. M.; ISHIDA, ROBSON KIYOSHI; GUARDA, ISMAEL FRANCISCO MOTA SIQUEIRA; DE MOURA, EDUARDO GUIMARAES HOURNEAUX; SAKAI, PAULO; SANTO, MARCO AURELIO; et al. Genetic reprogramming of rHemnant duodenum may contribute to type 2 diabetes improvement after Roux-en-Y gastric bypass. NUTRITION, v. 99-100, p. 10-pg., 2022-04-28. (13/03246-0, 11/09612-3, 14/07742-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

KOBAL, Priscila Sala. Changes in the gene expression of gastric and intestinal tissues of patients with tpe 2 diabetes mellitus submitted to Roux-en-Y gastric bypass. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.