Polyamines and ascorbate peroxidase as response rooting inductors Eucalyptus grandis x Eucalyptus urophylla

• Grant number: 13/09954-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2013
• End date: June 30, 2014
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Giuseppina Pace Pereira Lima
• Grantee: Roberta de Carvalho Dionizio
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Vegetative propagation is the main form of eucalyptus´s cuttings production for several advantages, however some clones still have difficulties in propagation, inclusively in rooting. Auxins are characterized as the main growth regulator able to improve this process. Indirectly, flavonoids and hydrogen peroxide can also contribute for rooting by increasing the activity of endogenous auxin. The aim of this work is verify hydrogen peroxide and quercetin, associates or not to auxin, effects in different rooting phases of Eucalyptus grandis x Eucalyptus urophylla, by biochemical markers. After 30, 60 and 90 days of plantation, height, diameter and survival rate will be determinate. Destructive analysis will be done, to determinate rooting percentage, number of roots, root length and ascorbate peroxidase activity, ascorbic acid and polyamines contents, biochemical markers of rooting. The determination of ascorbic acid will be done by titration, ascorbate peroxidase enzyme activity will be determined by spectrophotometric method and polyamines content will be done by thin layer chromatography. Data will be submitted to variance analysis (ANOVA) and means will be compared by Tukey test at 5% probability.