Influence of vitamin D receptor expression (VDR and TLR) in macrophages in the model of experimental diabetes mellitus

• Grant number: 12/23998-4
• Support type: Scholarships in Brazil - Master
• Effective date (Start): September 01, 2013
• Effective date (End): October 31, 2014
• Field of knowledge: Health Sciences - Pharmacy
• Principal researcher: Joilson de Oliveira Martins
• Grantee: Leonardo Mendes Bella
• Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 10/02272-0 - Effect of insulin on lung inflammation in animal with sepsis, innate immunit,activation of insulin gene (BGK) and insulin receptors (IR)-A and IR-B, AP.JP

Abstract

Vitamin D is a steroid hormone that acts in several mechanisms physiological (participating in the regulation of calcium homeostasis and immune system) and pathological (type 1 diabetes and tumors). Activation of vitamin D occurs through the effect of UVB rays on the skin with subsequent hydroxylations in liver and kidney. After activation, the interaction occurs with the Vitamin D Receptor (VDR) present within the cells, triggering their biological actions. Type 1 diabetes is an autoimmune disease characterized by the destruction of pancreatic beta (²) cells through the action of B lymphocytes, macrophages, CD4 + and CD8 + T cells that culminates in slow, progressive loss of insulin secretion. Vitamin D acts in different stages of the cascade of immune response. Studies have been demonstrated stimulating phagocytosis of the bacteria by macrophages and suppress the ability of antigen presenting dendritic cells, interfering with the production of interleukins (IL)-10, IL-12, tumor necrosis factor (TNF)-± and interferon (IFN)-³ and expression of Toll-like Receptors (TLR). The aim of this work is to study the relationship between vitamin D intake and hormonal mechanisms (production / release of insulin, PTH), blood glucose and the expression of receptors (VDR and TLR) of washed peritoneal macrophages (LPe) and bronchoalveolar (BAL) and immune response in healthy and diabetic mice. The knowledge of the effects of vitamin D in immunity and type 1 diabetes will allow a gain in quality of life of diabetic patients, allowing better control of the disease, since that hormone replacement therapy is easy to perform and inexpensive. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BELLA, LEONARDO M.; FIERI, ISIS; TESSARO, FERNANDO H. G.; NOLASCO, EDUARDO L.; NUNES, FERNANDA P. B.; FERREIRA, SABRINA S.; AZEVEDO, CAROLINA B.; MARTINS, JOILSON O. Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice. BIOMED RESEARCH INTERNATIONAL, 2017. Web of Science Citations: 2.

TESSARO, FERNANDO H. G.; AYALA, THAIS S.; NOLASCO, EDUARDO L.; BELLA, LEONARDO M.; MARTINS, JOILSON O. Insulin Influences LPS-Induced TNF-alpha and IL-6 Release Through Distinct Pathways in Mouse Macrophages from Different Compartments. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, v. 42, n. 5, p. 2093-2104, 2017. Web of Science Citations: 5.