Cytotoxicity of peracetic acid: evaluation of metabolism, structure, cell death and cell stress in L929 fibroblasts

• Grant number: 14/00723-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2014
• End date: March 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Gisele Faria
• Grantee: Kennia Scapin Viola
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Recently peracetic acid (PA) has been reported in the dental literature as a possible root canal irrigating solution. It has the advantage of bringing together in one product antibacterial activity and ability to remove smear layer. For the selection of root canal irrigating solution it should take into account not only its therapeutic benefits, but also the possible cytotoxic effects, since it can contact the periapical tissues. The aims of this study are: 1) To evaluate the cytotoxicity of the PA, at different concentrations, in comparison with sodium hypochlorite (NaOCl) through study of the cell metabolism in L929 fibroblasts; 2) To investigate the mechanism of cytotoxicity of AP in L929 fibroblasts through the evaluation of metabolism, structure, ultrastructure, cell death (necrosis and /or apoptosis) and stress of these cells. L929 fibroblasts in culture will be exposed to different concentrations of PA and NaOCl and to the culture medium for 24 hours (control). After this period, it will be evaluated the cell metabolism by means of metiltetrazólio assay (MTT), the external morphology of cells by scanning electron microscope, the ultrastructure by transmission electron microscope, the cytoskeleton by staining for actin and ±-tubulin, the type of cell death (necrosis or apoptosis) by flow cytometry and the cellular stress by marking of heat shock protein 70 (Hsp70). The statistical analysis will be performed using a significance level of 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIOLA, K. S.; RODRIGUES, E. M.; TANOMARU-FILHO, M.; CARLOS, I. Z.; RAMOS, S. G.; GUERREIRO-TANOMARU, J. M.; FARIA, G.. Cytotoxicity of peracetic acid: evaluation of effects on metabolism, structure and cell death. International Endodontic Journal, v. 51, p. 14-pg., 2018-05-01. (14/00723-5, 12/18203-2)

VIOLA, K. S.; RODRIGUES, E. M.; TANOMARU-FILHO, M.; CARLOS, I. Z.; RAMOS, S. G.; GUERREIRO-TANOMARU, J. M.; FARIA, G.. Cytotoxicity of peracetic acid: evaluation of effects on metabolism, structure and cell death. International Endodontic Journal, v. 51, n. 4, SI, p. E264-E277, MAY 2018. (12/18203-2, 14/00723-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VIOLA, Kennia Scapin. Cytotoxicity of peracetic acid: metabolic, structural and death evaluation in L929 fibroblasts. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.