Involvement of NO-dependent modifications in the control of Trypanosoma cruzi gene transcription throughout the adhesion of the parasite to the host extracellular matrix

• Grant number: 14/10046-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2014
• End date: July 31, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Anamaria Aranha Camargo
• Grantee: Rubens Daniel Miserani Magalhães
• Host Institution: Hospital Sírio-Libanês. Sociedade Beneficente de Senhoras (SBSHSL). São Paulo , SP, Brazil

Abstract

Chagas disease is a neglected illness with higher incidence in Latin America. Gene expression in T. cruzi is regulated mainly by post- transcriptional level, which emphasizes the importance of modifications in cromatin-associated proteins to control this process in trypanosomatids. The involvement of NO in the transcriptional regulation is well established in animal and plant cells and it is achieved by NO-dependent post-translational modifications of transcription factors or of secondary proteins related or not to epigenetic events. NO-dependent modifications of proteins from T. cruzi (nitration and S-nitrosylation) were recently described. Interestingly, an increment of nitration on tyrosine residues of histones (H2A and H4) was observed during the parasite adhesion to extracellular matrix, an important step in the parasite invasion, raising the hypothesis that NO may be involved in the control of gene expression in T. cruzi. In order to test this hypothesis, the ChIP-seq technique will be employed. ChIP-seq technique is an innovative methodology that uses next-generation sequencing to identify chromatin sequences associated with proteins of interest, generating maps of protein-DNA interactions. This project aims to identify the contribution of NO-dependent modifications of proteins related of the control of gene expression in T. cruzi using the ChIP-Seq technique. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MULE, S. N.; MANCHOLA, N. C.; DE OLIVEIRA, G. S.; PEREIRA, M.; MAGALHAES, R. D. M.; TEIXEIRA, A. A.; COLLI, W.; ALVES, M. J. M.; PALMISANO, G.. Proteome-wide modulation of S-nitrosylation in Trypanosoma cruzi trypomastigotes upon interaction with the host extracellular matrix. JOURNAL OF PROTEOMICS, v. 231, JAN 16 2021. (14/25494-9, 14/10046-0, 18/03727-2, 17/04032-5, 18/18257-1, 18/13283-4, 18/15549-1, 12/03887-3)

MISERANI MAGALHAES, RUBENS DANIEL; MATTOS, ELICIANE CEVOLANI; ROZANSKI, ANDREI; FAVORETTO GALANTE, PEDRO ALEXANDRE; PALMISANO, GIUSEPPE; CRUZ, ANGELA KAYSEL; COLLI, WALTER; CAMARGO, ANAMARIA ARANHA; MANSO ALVES, MARIA JULIA. Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix. PLoS Neglected Tropical Diseases, v. 14, n. 5, MAY 2020. (14/10046-0, 13/16002-2, 18/15549-1, 18/18257-1, 14/25494-9, 14/06863-3)