Rhinella schneideri mucous gland trascriptome

• Grant number: 14/15644-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2014
• End date: May 31, 2016
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Eliane Candiani Arantes Braga
• Grantee: Priscila Yumi Tanaka Shibao
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Natural products libraries are known as medicine molecules providers once these molecules have a high target specificity inherited from the long natural evolutionary process. Thereby, animal, plant and microorganisms' secretions are very important to biotechnological applications. However, despite the large number of molecules that can be found in those secretions, the difficulty on obtainment enough purification yield, high cost and long purification time, besides the small secretion amount provided by the studied organism, are factors that make this kind of study even harsher. Those are the reasons why omic studies are becoming an alternative to produce these toxins in a larger scale, in order to allow researches. Transcriptome, which consists on the production of cDNA libraries from the secretion or gland RNA, using the transcriptase reverse enzyme, is very important once it shows which proteins and peptides can be really found at the secretion expressed from animal DNA. Toads are animals that are still not widely studied, if we compare them with other venomous animals, mainly because of the insufficient purification yield. Thus, the mucous gland transcriptome from Rhinella schneideri poison, a toad that is widely found in Brazilian territory, has a great relevance on the elucidation and possibility to use this kind of molecules, especially because this gland produces unknown molecules. Finally, this study may provide an easier way to solve the purification yield problem besides the accomplishment to understand all the gland poison and provide the applicant a better knowledge for academic purposes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOLDRINI-FRANCA, JOHARA; COLOGNA, CAMILA TAKENO; PUCCA, MANUELA BERTO; FIGUEIREDO BORDON, KARLA DE CASTRO; AMORIM, FERNANDO GOBBI; PINO ANJOLETTE, FERNANDO ANTONIO; CORDEIRO, FRANCIELLE ALMEIDA; WIEZEL, GISELE ADRIANO; CERNI, FELIPE AUGUSTO; PINHEIRO-JUNIOR, ERNESTO LOPES; et al. Minor snake venom proteins: Structure, function and potential applications. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1861, n. 4, p. 824-838, APR 2017. (14/06170-8, 11/12317-3, 11/23236-4, 15/16714-8, 12/13590-8, 13/26619-7, 14/15644-3, 12/12954-6, 13/26200-6)

WIEZEL, GISELE A.; SHIBAO, PRISCILA Y. T.; COLOGNA, CAMILA T.; MORANDI FILHO, ROMUALDO; UEIRA-VIEIRA, CARLOS; DE PAUW, EDWIN; QUINTON, LOIC; ARANTES, ELIANE C.. In-Depth Venome of the Brazilian Rattlesnake Crotalus durissus terrificus: An Integrative Approach Combining Its Venom Gland Transcriptome and Venom Proteome. JOURNAL OF PROTEOME RESEARCH, v. 17, n. 11, p. 3941-3958, NOV 2018. (15/17466-8, 13/26200-6, 11/23236-4, 17/00586-6, 14/15644-3, 15/18432-0)

TANAKA SHIBAO, PRISCILA YUMI; COLOGNA, CAMILA TAKENO; MORANDI-FILHO, ROMUALDO; WIEZEL, GISELE ADRIANO; FUJIMURA, PATRICIA TIEMI; UEIRA-VIEIRA, CARLOS; ARANTES, ELIANE CANDIANI. Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 24, NOV 29 2018. (15/18432-0, 13/26200-6, 14/15644-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SHIBAO, Priscila Yumi Tanaka. Rhinella schneideri mucous gland transcriptome. 2016. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.