Study about Alphavirus and Flavivirus infections in samples of blood bank

• Grant number: 14/20851-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2015
• End date: March 31, 2019
• Field of knowledge: Biological Sciences - Microbiology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Luiz Tadeu Moraes Figueiredo
• Grantee: Marilia Farignoli Romeiro
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The arboviruses described to the Flaviviridae and Togaviridae families cause serious public health problems in tropical and subtropical countries. These arboviruses usually under diagnosed, involving clinical manifestations ranging from asymptomatic infection, acute febrile disease viral hemorrhagic fever and encephalitis. Due to the high number of asymptomatic infections and high viremia produced by Alphavirus and Flavivirus, blood donors could transmit these viruses by blood transfusion. Considering the recent introduction of Chikungunya (CHIKV) in Brazil, and the imminent introduction of West Nile virus (WNV), coupled with environmental factors such as climate and especially the urban high infestation by Aedes mosquitos, can be responsible for a large spread of these viruses the country. So CHIKV can cause serious epidemics, reproducing what is happening with the Dengue virus, transmitted by the same vector, which represents a serious risk to the safety of blood products. This study aims to detect Alphavirus and Flavivirus in blood bank samples using RT-PCR in real-time SYBR Green. In summary, this study will be important in transfusion service and also collaborate to improve the quality of blood products offered in Brazil. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NUNES-NETO, JOAQUIM PINTO; DE SOUZA, WILLIAM MARCIEL; ACRANI, GUSTAVO OLSZANSKI; ROMEIRO, MARILIA FARIGNOLI; FUMAGALLI, MARCILIOJORGE; VIEIRA, LUIZ CARLOS; DE ALMEIDA MEDEIROS, DANIELE BARBOSA; LIMA, JULIANA ABREU; SILVA DE LIMA, CLAYTON PEREIRA; CARDOSO, JEDSON FERREIRA; et al. Characterization of the Bujaru, frijoles and Tapara antigenic complexes into the sandfly fever group and two unclassified phleboviruses from Brazil. JOURNAL OF GENERAL VIROLOGY, v. 98, n. 4, p. 585-594, APR 2017. (12/24150-9, 16/01414-1, 14/20851-8, 15/05778-5, 13/14929-1, 14/02438-6)

COSTA BARREIRA, LUZIA APARECIDA; SCHEUCHER, PRISCILA SANTOS; ROMEIRO, MARILIA FARIGNOLI; LA SERRA, LEONARDO; BADRA, SORAYA JABUR; DE SOUZA, WILLIAM MARCIEL; MORAES FIGUEIREDO, LUIZ TADEU. Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells. Revista da Sociedade Brasileira de Medicina Tropical, v. 51, n. 2, p. 168-173, MAR-APR 2018. (17/13981-0, 12/24150-9, 14/02438-6, 14/20851-8)

ROMEIRO, MARILIA FARIGNOLI; DE SOUZA, WILLIAM MARCIEL; TOLARDO, ALINE LAVADO; VIEIRA, LUIZ CARLOS; HENRIQUES, DYANA ALVES; DE ARAUJO, JANSEN; HASSEGAWA SIQUEIRA, CARLOS EDUARDO; COLOMBO, TATIANA ELIAS; AQUINO, VICTOR HUGO; LOPES DA FONSECA, BENEDITO ANTONIO; et al. A real-time RT-PCR for rapid detection and quantification of mosquito-borne alphaviruses. ARCHIVES OF VIROLOGY, v. 161, n. 11, p. 3171-3177, NOV 2016. (13/02256-2, 12/02836-6, 13/14929-1, 12/24150-9, 14/20851-8)

DE SOUZA, WILLIAM MARCIEL; ROMEIRO, MARILIA FARIGNOLI; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; FUMAGALLI, MARCILIO JORGE; MODHA, SEJAL; TEIXEIRA NUNES, MARCIO ROBERTO; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. Novel orthohepeviruses in wild rodents from Sao Paulo State, Brazil. VIROLOGY, v. 519, p. 12-16, JUN 2018. (16/02568-2, 12/24150-9, 17/13981-0, 16/01414-1, 14/20851-8, 15/05778-5, 13/14929-1, 11/06810-9, 08/06411-4, 11/22663-6)

FUMAGALLI, MARCILIO JORGE; DE SOUZA, WILLIAM MARCIEL; ALVES ESPOSITO, DANILLO LUCAS; SILVA, ANGELICA; ROMEIRO, MARILIA FARIGNOLI; MARTINEZ, EDSON ZANGIACOMI; LOPES DA FONSECA, BENEDITO ANTONIO; MORAES FIGUEIREDO, LUIZ TADEU. Enzyme-linked immunosorbent assay using recombinant envelope protein 2 antigen for diagnosis of Chikungunya virus. VIROLOGY JOURNAL, v. 15, JUL 24 2018. (16/01414-1, 14/02438-6, 12/24150-9, 17/13981-0, 14/20851-8)

DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; DE ARAUJO, JANSEN; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONSALVES; ROMEIRO, MARILIA FARIGNOLI; MODHA, SEJAL; NARDI, MARCELLO SCHIAVO; QUEIROZ, LUZIA HELENA; DURIGON, EDISON LUIZ; et al. Discovery of novel anelloviruses in small mammals expands the host range and diversity of the Anelloviridae. VIROLOGY, v. 514, p. 9-17, JAN 15 2018. (16/02568-2, 12/24150-9, 16/01414-1, 14/20851-8, 15/05778-5, 13/14929-1, 11/06810-9, 08/06411-4, 11/22663-6)

ROMEIRO, MARILIA FARIGNOLI; FUMAGALLI, MARCILIO JORGE; DOS ANJOS SOUZA, ANA BEATRIZ; MORAES FIGUEIREDO, LUIZ TADEU. Serological evidence of Mayaro virus infection in blood donors from sao Carlos, sao Paulo, Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 114, n. 9, p. 693-696, SEP 2020. (16/01414-1, 14/02438-6, 14/20851-8)

DE SOUZA, WILLIAM MARCIE; FUMAGALLI, MARCILIO JORGE; TORRES CARRASCO, ADRIANO DE OLIVEIRA; ROMEIRO, MARILIA FARIGNOLI; MODHA, SEJAL; SEKI, MEIRE CHRISTINA; GHELLER, JANAINA MENEGAZZO; DAFFRE, SIRLEI; TEIXEIRA NUNES, MARCIO ROBERTO; MURCIA, PABLO RAMIRO; et al. Viral diversity of Rhipicephalus microplus parasitizing cattle in southern Brazil. SCIENTIFIC REPORTS, v. 8, NOV 5 2018. (16/01414-1, 14/02438-6, 15/05778-5, 13/14929-1, 12/24150-9, 17/13981-0, 14/20851-8)

FUMAGALLI, MARCILIO JORGE; DE SOUZA, WILLIAM MARCIEL; DE ARAUJO, JANSEN; MODHA, SEJAL; QUEIROZ, LUZIA HELENA; DURIGON, EDISON LUIZ; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. Krykfeie dicistrovirus: A novel dicistrovirus in velvety free-tailed bats from Brazil. INFECTION GENETICS AND EVOLUTION, v. 75, NOV 2019. (12/24150-9, 17/13981-0, 16/01414-1, 13/14929-1, 14/20851-8, 15/05778-5)

FUMAGALLI, MARCILIO JORGE; DE SOUZA, WILLIAM MARCIEL; ROMEIRO, MARILIA FARIGNOLI; DE SOUZA COSTA, MICHELL CHARLES; SLHESSARENKO, RENATA DEZENGRINI; MORAES FIGUEIREDO, LUIZ TADEU. Development of an Enzyme-Linked Immunosorbent Assay To Detect Antibodies Targeting Recombinant Envelope Protein 2 of Mayaro Virus. Journal of Clinical Microbiology, v. 57, n. 5, MAY 2019. (14/20851-8, 17/13981-0, 16/01414-1, 12/24150-9, 14/02438-6)

DE MELO JUNIOR, ARISTIDES BEZERRA; DE SOUZA, WILLIAM MARCIEL; ACRANI, GUSTAVO OLSZANSKI; CARVALHO, VALERIA LIMA; ROMEIRO, MARILIA FARIGNOLI; TOLARDO, ALINE LAVADO; DA SILVA, SANDRO PATROCA; CARDOSO, JEDSON FERREIRA; CHIANG, JANNIFER DE OLIVEIRA; DA SILVA GONCALVES VIANEZ JUNIOR, JOAO LIDIO; et al. Genomic characterization and evolution of Tacaiuma orthobunyavirus (Peribunyaviridae family) isolated in Brazil. INFECTION GENETICS AND EVOLUTION, v. 60, p. 71-76, JUN 2018. (16/17457-1, 14/20851-8, 17/13981-0, 12/24150-9, 14/02438-6)

DE SOUZA, WILLIAM MARCIEL; DENNIS, TRISTAN; FUMAGALLI, MARCILIO JORGE; ARAUJO, JANSEN; SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; ACRANI, GUSTAVO OLSZANSKI; TORRES CARRASCO, ADRIANO DE OLIVEIRA; ROMEIRO, MARILIA FARIGNOLI; MODHA, SEJAL; et al. Novel Parvoviruses from Wild and Domestic Animals in Brazil Provide New Insights into Parvovirus Distribution and Diversity. Viruses-Basel, v. 10, n. 4, APR 2018. (11/13821-7, 16/02568-2, 12/24150-9, 17/13981-0, 16/01414-1, 14/20851-8, 15/05778-5, 13/14929-1, 09/05994-9, 11/06810-9, 08/06411-4, 11/22663-6)

ROMEIRO, MARILIA FARIGNOLI; DE SOUZA, WILLIAM MARCIEL; TOLARDO, ALINE LAVADO; VIEIRA, LUIZ CARLOS; COLOMBO, TATIANA ELIAS; AQUINO, VICTOR HUGO; NOGUEIRA, MAURICIO LACERDA; MORAES FIGUEIREDO, LUIZ TADEU. Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 49, n. 3, p. 279-285, MAY-JUN 2016. (13/02256-2, 12/02836-6, 14/20851-8, 12/24150-9, 13/14929-1)

DE SOUZA, WILLIAM MARCIEL; ACRANI, GUSTAVO OLSZANSKI; ROMEIRO, MARILIA FARIGNOLI; REIS, JR., OSVALDO; TOLARDO, ALINE LAVADO; DA SILVA, SANDRO PATROCA; DE ALMEIDA MEDEIROS, DANIELE BARBOSA; VARELA, MARIANA; TEIXEIRA NUNES, MARCIO ROBERTO; MORAES FIGUEIREDO, LUIZ TADEU. Molecular characterization of Capim and Enseada orthobunyaviruses. INFECTION GENETICS AND EVOLUTION, v. 40, p. 47-53, JUN 2016. (14/20851-8, 12/24150-9, 15/05778-5, 13/14929-1, 14/02438-6)

DE SOUZA, WILLIAM MARCIEL; ROMEIRO, MARILIA FARIGNOLI; FUMAGALLI, MARCILIO JORGE; MODHA, SEJAL; DE ARAUJO, JANSEN; QUEIROZ, LUZIA HELENA; DURIGON, EDISON LUIZ; MORAES FIGUEIREDO, LUIZ TADEU; MURCIA, PABLO RAMIRO; GIFFORD, ROBERT JAMES. Chapparvoviruses occur in at least three vertebrate classes and have a broad biogeographic distribution. JOURNAL OF GENERAL VIROLOGY, v. 98, n. 2, p. 225-229, FEB 2017. (12/24150-9, 16/01414-1, 14/20851-8, 15/05778-5, 13/14929-1)

DE SOUZA, WILLIAM MARCIEL; ACRANI, GUSTAVO OLSZANSKI; ROMEIRO, MARILIA FARIGNOLI; REIS JUNIOR, OSVALDO; TOLARDO, ALINE LAVADO; SERRAO DE ANDRADE, AMANDA ARAUJO; GONCALVES VIANEZ JUNIOR, JOAO LIDIO DA SILVA; DE ALMEIDA MEDEIROS, DANIELE BARBOSA; TEIXEIRA NUNES, MARCIO ROBERTO; MORAES FIGUEIREDO, LUIZ TADEU. Complete genome sequence of Piry vesiculovirus. ARCHIVES OF VIROLOGY, v. 161, n. 8, p. 2325-2328, AUG 2016. (12/24150-9, 13/14929-1, 14/20851-8, 13/02256-2, 14/02438-6, 15/05778-5)

DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; MARTIN, MATHEUS CAVALHEIRO; DE ARAUJO, JANSEN; ORSI, MARIA ANGELA; SANFILIPPO, LUIZ FRANCISCO; MODHA, SEJAL; DURIGON, EDISON LUIZ; PROENCA-MODENA, JOSE LUIZ; ARNS, CLARICE WEIS; et al. Pingu virus: A new picornavirus in penguins from Antarctica. VIRUS EVOLUTION, v. 5, n. 2, JUL 2019. (17/13981-0, 12/24150-9, 16/01414-1, 13/14929-1, 14/20851-8, 15/05778-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROMEIRO, Marilia Farignoli. A study on alphavirus and flavivirus infections in blood bank samples. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.