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Influence of miR-10b on proliferation and the invasive potential of osteosarcoma

Grant number: 15/00524-5
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2015
Effective date (End): June 30, 2017
Field of knowledge:Health Sciences - Medicine
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:María Sol Brassesco Annichini
Grantee:Gabriela Molinari Roberto
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Osteosarcoma is the most common pediatric bone tumor. It is found mainly in the metaphysis of long bones. The current treatment consists on surgical tumor removal and multimodal neoadjuvant and adjuvant chemotherapy. About a third of patients are affected by post-surgical recurrence and distant metastases on lungs and bones. Only 20% of the patients with metastases remain alive after 5 years. Therefore, it is essential to elucidate the molecular pathways involved in the invasive and metastatic process of tumor type. Several miRNAs have been suggested as regulators of the metastatic pathways, including the miR10b. This microRNA is overexpressed in numerous metastatic tumor types. It acts on the invasive processes through an incomplete pairing with the messenger RNA of the HOXD10 gene inhibiting its translation. The HOXD10 is characterized as a general inhibitor of invasion acting on different molecules including the Rho GTPases RhoA and RhoC. These enzymes phosphorylate and activate ROCK1 and ROCK2 kinases that are responsible for the control of the cytoskeleton, an essential mechanism for the cell invasion processes. Furthermore, studies suggest that ROCKS act on cell proliferation, apoptosis and cell cycle control. Many studies have reported overexpression of these kinases in several malignancies, including osteosarcoma. Thus, this study aims to analyze the expression profile of miR10b in osteosarcoma, as well as elucidating its role in the proliferative and invasive processes of this type of cancer. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROBERTO, GABRIELA MOLINARI; VIEIRA, GABRIELA MACIEL; ALBERICI DELSIN, LARA ELIS; SILVA, MARCELA DE OLIVEIRA; HAKIME, RODRIGO GUEDES; ENGEL, EDGARD EDUARD; SCRIDELI, CARLOS ALBERTO; TONE, LUIZ GONZAGA; BRASSESCO, MARIA SOL. MiR-708-5p is inversely associated with EWS/FLI1 Ewing sarcoma but does not represent a prognostic predictor. CANCER GENETICS, v. 230, p. 21-27, JAN 2019. Web of Science Citations: 2.
ROBERTO, GABRIELA MOLINARI; ENGEL, EDGARD EDUARD; SCRIDELI, CARLOS ALBERTO; TONE, LUIZ GONZAGA; BRASSESCO, MARIA SOL. Downregulation of miR-10B{*}is correlated with altered expression of mitotic kinases in osteosarcoma. PATHOLOGY RESEARCH AND PRACTICE, v. 214, n. 2, p. 213-216, FEB 2018. Web of Science Citations: 5.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ROBERTO, Gabriela Molinari. Influence of miR-10b on proliferation and the invasive potential of osteosarcoma. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto Ribeirão Preto.

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