FGF2, VEGF, DSPP, and DMP-1 expression in cells of the pulp deciduous teeth with a low intensity laser

• Grant number: 15/19696-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2016
• End date: February 28, 2017
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Thais Marchini de Oliveira Valarelli
• Grantee: Mariel Tavares de Oliveira Prado Bergamo
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The objective of this study is to investigate the Low Intensity Laser effects of different energy densities on viability of the pulp of human deciduous teeth; mRNA expression of FGF2, VEGF, DSPP and DMP-1; and expression of the FGF2, VEGF, DSPP and DMP-1 protein. Human deciduous teeth samples of pulp tissue will be obtained and grown in specific culture. After the fourth passage, the sample will be irradiated with low intensity laser to the following groups: Group I - Ia (1.2 J/cm2 - 05 mW - 10s), Ib (2.5 J/cm2 - 05 mW - 20s), Ic (3.7 J/cm2 - 05 mW - 30s), Id (5.0 J/cm2 - 05 mW - 40s), ie (6.2 J/cm2 - 05 mW - 50s), if (no irradiated); Group II - IIa (1.2 J/cm2 - 05 mW - 10s), IIb (2.5 J/cm2 - 10 mW - 10s), IIc (3.7 J/cm2 - 20 mW - 10s), IId ( 5.0 J/cm2 - 30 mW - 10 sec), IIe (6.2 J/cm2 - 40 mW - 10 sec), IIF (no irradiated). The techniques used for the analysis will be MTT, Crystal Violet, RT-PCR and ELISA. The values in triplicate will be analyzed by ANOVA followed by Tukey test and Kruskal-Wallis analysis complemented by Dunn's test when obey normal and non-normal distribution, respectively. The significance level of 5% will be adopted for the differences considered statistically significant. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA PRADO, MARIE TAVARES; RIBEIRO VITOR, LUCIANA LOURENGO; LOURENCO NETO, NATALINO; TEIXEIRA MARQUES, NADIA CAROLINA; SAKAI, VIVIEN THIEMY; RIOS, DANIELA; CRUVINEL, THIAGO; OLIVEIRA, RODRIGO CARDOSO; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; et al. Effects of different culture media, cell densities and adhesion periods on stem cells from human exfoliated deciduous teeth after photobiomodulation. LASER PHYSICS LETTERS, v. 16, n. 9, SEP 2019. (15/19696-0, 13/18886-5)

TEIXEIRA MARQUES, NADIA CAROLINA; LOURENCO NETO, NATALINO; OLIVEIRA PRADO, MARIEL TAVARES; RIBEIRO VITOR, LUCIANA LOURENCO; OLIVEIRA, RODRIGO CARDOSO; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; OLIVEIRA, THAIS MARCHINI. Effects of PBM in different energy densities and irradiance on maintaining cell viability and proliferation of pulp fibroblasts from human primary teeth. Lasers in Medical Science, v. 32, n. 7, p. 1621-1628, SEP 2017. (13/16156-0, 13/18886-5, 15/19696-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BERGAMO, Mariel Tavares de Oliveira Prado. FGF2, VEGF, DSPP, and DMP-1 expression in cells of the pulp deciduous teeth with a Low Intensity Laser. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.