| Grant number: | 16/07936-0 |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| Start date: | June 30, 2016 |
| End date: | December 29, 2016 |
| Field of knowledge: | Biological Sciences - Microbiology - Applied Microbiology |
| Principal Investigator: | Susana Marta Isay Saad |
| Grantee: | Marina Padilha |
| Supervisor: | Karsten Kristiansen |
| Host Institution: | Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Institution abroad: | University of Copenhagen, Copenhagen, Denmark |
| Associated to the scholarship: | 13/26435-3 - Impact of the maternal diet and the intervention with fructooligosaccharide on the human milk microbiota., BP.DR |
Abstract Human milk is recognized as the main component for growth, metabolism, and immune development in infants. Furthermore, during lactation, human milk is an important source of microorganisms for the intestinal colonization of newborns. Mother-related factors have been associated with the human milk microbiota composition. Nevertheless, apparently, there has not been any study in which the maternal diet was evaluated as a modulator of the human milk microbiota. Therefore, the aim of this study is to investigate the impact of the maternal diet on the human milk microbiota composition of healthy women on breastfeeding practice, and subsequently, to evaluate the effect of fructooligosaccharides supplementation on the human milk microbiota. In order to investigate a possible association between macronutrients (carbohydrates, lipids, and proteins) and fibers intake with the human milk microbiota, a clinical trial will be divided in two parts. The first part will consist of a cross-sectional study with 100 lactating women, recruited at the University Hospital of University of São Paulo (HU/USP). The second part will consist of a randomized, placebo-controlled clinical trial with 60 lactating from the first part of the clinical trial. The participants will be divided into groups of supplementation and placebo group, which will receive 4.5 g of fructooligosaccharides + 2 g of maltodextrin or 2 g of maltodextrin, respectively, over a period of 20 days. The dietary assessment will be estimated by the 24 hour recall method (24HRM) and the Quantitative Food Frequency Questionnaire (QFFQ) for use in pregnancy. The microbiota in human milk will be analyzed from milk samples aseptically collected and DNA will be extracted by phenol:chloroform with ethanol precipitation. The DNA obtained will be used as template for amplification and sequencing by the Illumina MiSeq® System. (AU) | |
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