The influence of niobium (Nb) and zirconium (Zr) elements present in the alloy Ti-Nb-Zr about osteoblastic activity and biofilm formation: comparative study Ti puro

• Grant number: 16/07768-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2016
• End date: July 31, 2017
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Luana Marotta Reis de Vasconcellos
• Grantee: Thais Fernanda Gonçalves
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Adequate corrosion resistance and the shear, the low modulus of elasticity and biocompatibility are relevant characteristics of the new Titanium alloys, making it humans upper material is very promising for orthopedic implants. The objective this study will be to evaluate in vitro the influence of Niobium (Nb) and Zirconium (Zr) elements present in the alloy Ti-Nb-Zr about osteoblastic activity and biofilm formation. The samples will be make with different materials and then they will be divide: a) titanium(Ti - Control); b) Ti-35Nb-7Zr; c) Nb and d) Zr. Previously the in vitro study, all the samples will be identifie by scanning electron microscopy and energy dispersive spectroscopy to observe the surface topography and presence of elements. Osteoblastic cells, differentiated mesenchymal cells obtained from femurs rats will be grown on samples and after predetermined periods, will be make cytotoxicity tests, alkaline phosphatase activity and quantification of the nodule. Analysis for formation of biofilms, standardize suspensions (107 cells / ml) will be add in microplate wells containing as samples. Then the monotypic biofilms of S. aureus, S. mutans, S.sanguinis will be broke with ultrasonic homogenizer, and the suspensions will be dilute and plate on agar Brain Heart Infusion (BHI). After 48 hours, the colonies forming units will be count for milliliter (CFU / mL) and values obtain will be convert to log10.All data obtain will be analyze using the normal test and then the statistical tests will beapplies with appropriate level of significance of 5%.