Analysis of gene expression of TLR4 and TNF-± in obese rats myocardial

• Grant number: 16/13592-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): October 01, 2016
• Effective date (End): September 30, 2019
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Camila Renata Corrêa
• Grantee: Pedro Henrique Rizzi Alves
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Obesity is defined as an excessive accumulation of body fat that can harm health. Excess adipose tissue is accompanied by a state of chronic low-grade inflammation that affects many organs, including the heart. Because of the importance of this fact, some mechanisms are identified as trigger of inflammation in obesity, for example, toll-like- transmembrane receptor 4 (TRL-4). The TLR4 from a family of receptors that play a role in the detection and recognition of microbial pathogens is involved in the immunological response recognize the lipopolysaccharide (LPS) from the cell wall of bacteria. Many studies report that these receptors play a critical relation mediating the inflammatory response in heart disease, but it is not our knowledge works that relate the expression of these receptors in the heart in obese animals. This study aims to test the influence of chronic supply of sugar and saturated fat on gene expression of Toll like receptor 4 in the myocardium. They will be used male Wistar rats (n = 16), 21 days old, divided into two experimental groups (n = 8 animals / group). The G1 group will receive standard diet, G2 high-carbohydrate diet (HCHO) for 30 weeks. The consumption of diets and drink intake will be monitored daily. The fat index will be used as an indicator of obesity, since it allows to accurately assess the amount of body fat of animals. Plasma samples will be used for the serum glucose determinations, and uric acid triglyceride using colorimetric enzymatic method. The morphology and cardiac function will be evaluated in vivo by ecorcardiografia transthoracic. The RNA is subjected to reverse transcription, RNA translation in complementary deoxyribonucleic acid (cDNA) and used in polymerase chain reaction (PCR) using ready assays (Applied Biosystems, CA, USA) containing probe TaqMan MGB (FAM) and specific primers.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEDRO HENRIQUE RIZZI ALVES; ARTUR JUNIO TOGNERI FERRON; MARIANE RÓVERO COSTA; FABIANA KUROKAWA HASIMOTO; CRISTINA SCHMITT GREGOLIN; JÉSSICA LEITE GARCIA; DIJON HENRIQUE SALOMÉ DE CAMPOS; ANTÔNIO CARLOS CICOGNA; LETÍCIA DE MATTEI; FERNANDO MORETO; et al. Relação entre Resposta Imune Inata do Receptor Toll-Like-4 (TLR-4) e o Processo Fisiopatológico da Cardiomiopatia da Obesidade. Arquivos Brasileiros de Cardiologia, v. 117, n. 1, p. 91-99, 2021-07-26. (15/10626-0, 16/13592-1)