Extraction of bioactive compounds from husks of pomegranate (Punica granatum L.) using pressurized liquids assisted by ultrasound

• Grant number: 16/20016-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2016
• End date: December 03, 2017
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Maurício Ariel Rostagno
• Grantee: Mariana Pacífico dos Santos
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 13/04304-4 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology, AP.JP

Abstract

The rind of pomegranate (Punica granatum L.) are rich raw material in bioactive compounds, such as punicalagins and ellagic acid, which can be extracted using the appropriate techniques and conditions. This research project will evaluate the possibility of assisting the extraction with pressurized liquid with ultrasound to improve the efficiency, selectivity and yield of the process for the isolation of these compounds from pomegranate rind. Due to the influence of process variables in the propagation of ultrasonic waves and cavitation several variables will be evaluated to determine the adequacy of combining ultrasound with pressurized liquid extraction. To maximize the effect of ultrasound application in the extraction process the use of different amounts of carbon dioxide as expansion gas will be evaluated seeking to enhance the cavitation phenomenon. The ethyl lactate will be evaluated as an alternative extraction solvent seeking a process that is not only efficient but also environmentally friendly. Initially the combined effects of temperature (60-120 ° C), solvent extraction (25-75% ethyl lactate in water and 70% ethanol in water), and ultrasound power (0-800 W) will be studied. In a second step interactions between the amount of expansion gas (0-10 wt%), pressure (50-350 bar) and ultrasound power will be evaluated. Finally, different extraction times (10-30 minutes) and number of extraction cycles (cycles 1-3) will be evaluated. The results will be analyzed based on the yield of dry extract, concentration of bioactive compounds by liquid chromatography, total polyphenol content and antioxidant activity by two different methods (FRAP and TEAC). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, MARIANA P.; SOUZA, MARIANA C.; SUMERE, BEATRIZ R.; DA SILVA, LAISE C.; CUNHA, DIOGO T.; BEZERRA, ROSANGELA MARIA N.; ROSTAGNO, MAURICIO A.. Extraction of bioactive compounds from pomegranate peel (Punica granatum L.) with pressurized liquids assisted by ultrasound combined with an expansion gas. ULTRASONICS SONOCHEMISTRY, v. 54, p. 11-17, JUN 2019. (16/19930-6, 16/20016-7)

SUMERE, BEATRIZ ROCCHETTI; DE SOUZA, MARIANA CORREA; DOS SANTOS, MARIANA PACIFICO; NEVES BEZERRA, ROSANGELA MARIA; DA CUNHA, DIOGO THIMOTEO; MARTINEZ, JULIAN; ROSTAGNO, MAURICIO ARIEL. Combining pressurized liquids with ultrasound to improve the extraction of phenolic compounds from pomegranate peel (Punica granatum L.). ULTRASONICS SONOCHEMISTRY, v. 48, p. 151-162, NOV 2018. (16/19930-6, 15/11932-7, 16/06457-0, 16/20016-7)

SOUZA, MARIANA C.; SANTOS, MARIANA P.; SUMERE, BEATRIZ R.; SILVA, LAISE C.; CUNHA, DIOGO T.; MARTINEZ, JULIAN; BARBERO, GERARDO F.; ROSTAGNO, MAURICIO A.. Isolation of gallic acid, caffeine and flavonols from black tea by on-line coupling of pressurized liquid extraction with an adsorbent for the production of functional bakery products. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 117, JAN 2019. (16/20016-7, 16/19930-6, 18/17089-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Mariana Pacífico dos. Extraction of bioactive compounds from the pomegrante shell (Punica granatum L.) using the combination of pressurized ultrasound-assisted liquids associated with the nitrogen expansion gas, with application of the extract in an obesity induction model. 2018. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas Limeira, SP.