Bioactive metabolites from Nectandra oppositifolia Nees & Mart. (Lauraceae): molecular characterization, in vitro and in vivo antiparasitic potential evaluation and determination of mechanism of action

• Grant number: 16/20633-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2017
• End date: December 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: João Henrique Ghilardi Lago
• Grantee: Geanne Alexsandra Alves Conserva
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated scholarship(s): 18/18975-1 - Dereplication profile of Nectandra oppositifolia Nees & Mart. (Lauraceae): identification and molecular characterization of antiparasitic metabolites, BE.EP.DR

Abstract

Plants are used for medicinal purposes since ancient times. Natural compounds extracted from plants have been highlighted as important sources of new therapeutic agents, used in the treatment of several ills, especially neglected diseases, such as leishmaniosis, Chagas disease among others. In this aspect, several studies are directed to evaluation of biological activities of natural compounds from plants, as well as investigation of mechanism of action of potential bioactive compounds. In this context, this project aims the isolation, purification and structural identification and/or elucidation of compounds with antiparasitic activity (antileishmanial and trypanocidal) from extracts of leaves and branches of Nectandra oppositifolia Ness & Mart. (Lauraceae), specie still unknown in chemical point of view. Therefore, a phytochemical dereplication study will be conducted, followed by a bioactivity-guided fractionation, aiming the isolation and structural characterization of compounds present in the active extracts, which will be re-evaluated against those activities observed for crude extracts. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONSERVA, GEANNE A.; COSTA-SILVA, THAIS A.; QUIROS-GUERRERO, LUIS M.; MARCOURT, LAURENCE; WOLFENDER, JEAN-LUC; QUEIROZ, EMERSON F.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Kaempferol-3-O-alpha-(3,4-di-E-p-coumaroyl)-rhamnopyranoside from Nectandra oppositifolia releases Ca2+from intracellular pools of Trypanosoma cruzi affecting the bioenergetics system. Chemico-Biological Interactions, v. 349, NOV 1 2021. (16/20633-6, 21/04464-8, 21/02789-7, 18/18975-1)

MORAIS, THIAGO R.; ALVES CONSERVA, GEANNE A.; VARELA, MARINA T.; COSTA-SILVA, THAIS A.; THEVENARD, FERNANDA; PONCI, VITOR; FORTUNA, ANA; FALCAO, AMILCAR C.; TEMPONE, ANDRE G.; FERNANDES, JOAO PAULO S.; et al. Improving the drug-likeness of inspiring natural products - evaluation of the antiparasitic activity against Trypanosoma cruzi through semi-synthetic and simplified analogues of licarin A. SCIENTIFIC REPORTS, v. 10, n. 1, MAR 25 2020. (16/20633-6, 16/25028-3, 18/10279-6, 18/07885-1)

ROSA, MATHEUS ELIAS; CONSERVA, GEANNE A. ALVES; PASSERO, LUIZ FELIPE D.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Unsaturated lipids modulating the interaction of the antileishmanial isolinderanolide E with models of cellular membranes. BIOORGANIC CHEMISTRY, v. 124, p. 7-pg., 2022-07-01. (21/02789-7, 19/04407-4, 16/20633-6, 18/22214-6, 18/24077-6, 19/03239-0)

DA COSTA-SILVA, THAIS A.; ALVES CONSERVA, GEANNE A.; GALISTEO, JR., ANDRES J.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae). Journal of Venomous Animals and Toxins including Tropical Diseases, v. 25, AUG 19 2019. (16/20633-6, 18/10279-6, 18/07885-1)

ALVES CONSERVA, GEANNE A.; DA COSTA-SILVA, THAIS A.; AMARAL, MAIARA; ANTAR, GUILHERME M.; NEVES, BRUNO J.; ANDRADE, CAROLINA H.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Butenolides from Nectandra oppositifolia (Lauraceae) displayed anti-Trypanosoma cruzi activity via deregulation of mitochondria. Phytomedicine, v. 54, p. 302-307, FEB 15 2019. (16/20633-6, 15/23403-9, 18/07885-1)

ROSA, MATHEUS ELIAS; ALVES CONSERVA, GEANNE A.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. nteraction of isolinderanolide E obtained from Nectandra oppositifolia with biomembrane model. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1863, n. 10, OCT 2021. (19/03239-0, 16/20633-6, 18/22214-6, 19/04407-4)

ALVES CONSERVA, GEANNE A.; QUIROS-GUERRERO, LUIS M.; COSTA-SILVA, THAIS A.; MARCOURT, LAURENCE; PINTO, ERIKA G.; TEMPONE, ANDRE G.; FERNANDES, JOAO PAULO S.; WOLFENDER, JEAN-LUC; QUEIROZ, EMERSON F.; LAGO, JOAO HENRIQUE G.. Metabolite profile of Nectandra oppositifolia Nees & Mart. and assessment of antitrypanosomal activity of bioactive compounds through efficiency analyses. PLoS One, v. 16, n. 2, FEB 25 2021. (18/18975-1, 18/07885-1, 16/20633-6, 18/10279-6, 15/23403-9)

ROSA, MATHEUS ELIAS; ALVES CONSERVA, GEANNE A.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Monolayer nanoarchitectonics at the air-water interface for molecular understanding of the interaction of isolinderanolide E with cholesterol. Thin Solid Films, v. 754, p. 7-pg., 2022-07-31. (19/03239-0, 16/20633-6, 18/22214-6, 21/02789-7, 19/04407-4)

CONSERVA, G. A. A.; MORAIS, T. R.; RODRIGUES, V. C.; DE MORAES, J.; LAGO, J. H. G.. In vivo anthelmintic activity evaluation of licarin A, a neolignan isolated from leaves of Nectandra oppositifolia (Lauraceae). Planta Medica, v. 85, n. 18, p. 2-pg., 2019-12-01. (16/20633-6, 18/18975-1)