Purification and immobilization of beta-glucosidase from Thermoascus aurantiacus using supermacroporous cryogels and application in bioprocesses

• Grant number: 16/17812-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2017
• End date: June 30, 2019
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Roberto da Silva
• Grantee: Paula Chequer Gouveia Mol
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

beta-glucosidase are enzymes that hydrolyze terminal beta-(1.4) glycoside bonds, essential function to many biological processes, being of great interest to biochemistry and biotechnology. This study involves the extraction and subsequent purification of ²-glucosidase of thermophilic fungus Thermoascus aurantiacus in a supermacroporous support of polyacrylamide produced in cryogenic conditions and chemically modified by the introduction of ionic groups N, N-dimethylaminoethyl methacrylamide. For this, different pH values and different flow rates of the mobile phase will be tested on the specific activity of the purified enzyme. After the purification step, an immobilization procedure will be studied using the same support. The effects of pH and temperature on the stability of free and immobilized enzymes will be determined, as well as the determination of the kinetic parameters of Michaelis-Menten model. Finally, the performance of the system containing the immobilized enzymes will be tested in the hydrolysis of isoflavone and degradation of pretreated sugar cane bagasse. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOUVEIA MOL, PAULA CHEQUER; ALCANTARA VERISSIMO, LIZZY AYRA; MINIM, LUIS ANTONIO; BOSCOLO, MAURICIO; GOMES, ELENI; DA SILVA, ROBERTO. Production and capture of beta-glucosidase from Thermoascus aurantiacus using a tailor made anionic cryogel. Process Biochemistry, v. 82, p. 75-83, JUL 2019. (17/16482-5, 16/17812-6)

GOUVEIA MOL, PAULA CHEQUER; ALCANTARA VERISSIMO, LIZZY AYRA; MINIM, LUIS ANTONIO; DA SILVA, ROBERTO. Adsorption and immobilization of beta-glucosidase from Thermoascus aurantiacus on macroporous cryogel by hydrophobic interaction. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. N/A, p. 11-pg., 2022-06-03. (16/17812-6, 17/16482-5)

MOL, PAULA CHEQUER GOUVEIA; QUILLES, JOSE CARLOS; VERISSIMO, LIZZY AYRA ALCANTARA; BOSCOLO, MAURICIO; GOMES, ELENI; MINIM, LUIS ANTONIO; DA SILVA, ROBERTO. B-glucosidase: An overview on immobilization and some aspects of structure, function, applications and cost. Process Biochemistry, v. 130, p. 14-pg., 2023-04-05. (17/16482-5, 16/17812-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MOL, Paula Chequer Gouveia. Purification and immobilization of β-glucosidase from the fungus Thermoascus aurantiacus in supermacroporous cryogel. 2021. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.