Proteolytic processing analysis in the secretome of melanoma tumor cells

• Grant number: 17/07897-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2017
• End date: July 31, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: André Zelanis Palitot Pereira
• Grantee: Isabella Fukushima
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Federal de São Paulo (UNIFESP). Campus São José dos Campos. São José dos Campos , SP, Brazil
• Associated research grant: 14/06579-3 - System-wide analysis of N-terminal processing and protein diversity in the secretome of tumor cells, AP.JP
• Associated scholarship(s): 17/24560-6 - Proteolytic processing analysis in the secretome of melanoma tumor cells, BE.EP.IC

Abstract

The search for biomarkers has been addressed by several investigators in the field of cancer biology, however, given the multitude of etiologic factors associated to oncogenesis, a number of challenges still need to be overcome. The imbalance of cellular homeostasis during oncogenesis has a marked effect on the repertoire of the proteins secreted by malignant cells (cellular secretome), as well as, upon the physiological processing events that occurs during or after protein synthesis. The development and improvement of high throughput analytical techniques provide main evidences regarding the complexity in ordinary physiological mechanisms of eukaryotic cells, as well as pathological processes such as cancer. In this context, proteolytic processing is responsible for the generation of new protein N-termini and comprises an essentially irreversible cell signaling process, affecting a number of biological pathways with important implications in oncogenesis. Therefore, the main goal of this project is to identify the peptidome diversity derived from proteolytic processing events in the secretome of cultured tumor cells correlating the results with biological pathways that might be eventually involved in the oncogenesis process. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZELANIS, ANDRE; SILVA, DEBORA A.; KITANO, EDUARDO S.; LIBERATO, TARCISIO; FUKUSHIMA, ISABELLA; SERRANO, SOLANGE M. T.; TASHIMA, ALEXANDRE K.. A first step towards building spectral libraries as complementary tools for snake venom proteome/peptidome studies. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 31, SEP 2019. (13/14651-3, 14/06579-3, 17/20106-9, 13/07467-1, 17/07897-7, 13/13548-4)

LIBERATO, TARCISIO; PESSOTTI, DAYELLE S.; FUKUSHIMA, ISABELLA; KITANO, EDUARDO S.; SERRANO, SOLANGE M. T.; ZELANIS, ANDRE. Signatures of protein expression revealed by secretome analyses of cancer associated fibroblasts and melanoma cell lines. JOURNAL OF PROTEOMICS, v. 174, p. 1-8, MAR 1 2018. (13/07467-1, 14/06579-3, 17/07897-7)

LIBERATO, TARCISIO; FUKUSHIMA, ISABELLA; KITANO, EDUARDO S.; SERRANO, SOLANGE M. T.; CHAMMAS, ROGER; ZELANIS, ANDRE. Proteomic profiling of the proteolytic events in the secretome of the transformed phenotype of melanocyte-derived cells using Terminal Amine Isotopic Labeling of Substrates. JOURNAL OF PROTEOMICS, v. 192, p. 291-298, FEB 10 2019. (14/06579-3, 13/07467-1, 17/07897-7)