Development of Gelatin-Chitosan based scaffolds for wound healing and skin regeneration: in vitro and in vivo assessment

• Grant number: 16/24209-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): May 01, 2017
• Effective date (End): April 30, 2019
• Field of knowledge: Interdisciplinary Subjects
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Paulo José do Amaral Sobral
• Grantee: Daniel Enrique López Angulo
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated research grant: 13/07914-8 - FoRC - Food Research Center, AP.CEPID

Abstract

Serious trauma, genetic skin disorders and chronic wounds might result in skin loss, with burns and scalds being major contributors to rapid, extensive, deep wounds with substantial areas of skin damage, frequently without the possibility of skin regeneration. Scaffold plays a critical role in the success of the living tissue reconstructs damaged by such traumas. Enhancement of cellular attachment, proliferation, and the organized development of native structures are all the attributes of a beneficial scaffold. This study provides a multidisciplinary work between the area of veterinary medicine and tissue engineering applied to biomedical aplications. The area of veterinary medicine, currently provides valuable tools to evaluate the performance of these materials regarding their interaction with cells responsible for healing, proliferation, epithelialization and tissue repair. The potential for use of these scaffolds for tissue regeneration will be evaluated in vitro using rabbit fibroblasts and messenchimal cells, in which the cell adhesion, proliferation, and morphology will be investigated. The scaffold constructs based Gelatin-Chitosan and Aloe vera and Snail mucus will be examined by SEM, microcomputed tomography (micro CT), histological analysis, in vitro cell viability study and in vivo assessment. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZAMUDIO, RAPHAELA MELLO; PEREIRA, VITORIA MATTOS; ANGULO, DANIEL ENRIQUE LOPEZ; AMBROSIO, CARLOS EDUARDO; LOURENCO, RODRIGO VINICIUS; MARTINS, DANIELE DOS SANTOS; AKASHI, LARISSA; BITTANTE, ANA MONICA Q. B.; SOBRAL, PAULO JOSE DO AMARAL. Obtaining a freeze-dried biomaterial for skin regeneration: Reinforcement of the microstructure through the use of crosslinkers and in vivo application. Materials Chemistry and Physics, v. 290, p. 12-pg., 2022-08-06. (17/19682-5, 16/24209-4)

LOPEZ ANGULO, DANIEL ENRIQUE; AMBROSIO, CARLOS EDUARDO; LOURENCO, RODRIGO; NARDELLI GONCALVES, NATALIA JULIANA; CURY, FABIO SERGIO; DO AMARAL SOBRAL, PAULO JOSE. Fabrication, characterization and in vitro cell study of gelatin-chitosan scaffolds: New perspectives of use of aloe vera and snail mucus for soft tissue engineering. Materials Chemistry and Physics, v. 234, p. 268-280, AUG 1 2019. (16/24209-4, 13/07914-8)