Optimization of cello-oligosaccharides production by enzymatic hydrolysis of sugarcane straw via heterologous expression of cellulolytic and oxidative enzymes

• Grant number: 17/16159-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: January 01, 2018
• End date: October 31, 2020
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Rosana Goldbeck Coelho
• Grantee: Fernando César Barbosa
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks, AP.TEM
• Associated scholarship(s): 19/02214-4 - Engineering and optimization of expression systems for intrinsically disordered proteins, BE.EP.DD

Abstract

Bioethanol has been considered the main alternative to the crescent world demand for fuel because it is capable of reducing the environment impacts caused by non-renewable fuels, such as increased pollution and global warming. Based on that, the sugarcane straw represent a potential source of lignocellulosic biomass for 2nd generation bioethanol production, since it can represent more than 25 % of all the ethanol produced in Brazil per year. In addition, the enzymatic conversion of the lignocellulosic biomass in cello-oligosaccharides instead of saccharides is a field still under-researched. It may be an interesting alternative to the traditional transformation of biomass in glucose given its advantages, such as lower process contaminants, shorter total fermentation time, higher binding affinity to cellulosic substrates, and limited process inhibition by high glucose concentration. With this premise, this project has the main goal of optimize the production of cello-oligosaccharides by enzymatic hydrolysis of pretreated sugarcane straw via heterologous expression of cellulolytic and oxidative enzymes. For this, this project also aims to identify the key enzymes (cellulolytic and oxidative) required for degradation of pretreated straw (cellolignin) in cello-oligosaccharides; select interesting genes, clone and express in E.coli, as well as purify and test activity/function of novel enzyme candidates having key activities. Moreover, it will be performed the optimization of cello-oligosaccharides production by experimental designs of key enzymes mixtures. Finally, we expect to obtain a hydrolyzed rich in cello-oligosaccharides for further fermentation by Geobaccillus. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARBOSA, FERNANDO CESAR; SILVELLO, MARIA AUGUSTA; GOLDBECK, ROSANA. Cellulase and oxidative enzymes: new approaches, challenges and perspectives on cellulose degradation for bioethanol production. Biotechnology Letters, v. 42, n. 6, p. 875-884, JUN 2020. (18/20787-9, 19/08542-3, 17/16159-0)

BARBOSA, FERNANDO CESAR; KENDRICK, EMANUELE; BRENELLI, LIVIA BEATRIZ; ARRUDA, HENRIQUE SILVANO; PASTORE, GLAUCIA MARIA; RABELO, SARITA CANDIDA; DAMASIO, ANDRE; FRANCO, TELMA TEIXEIRA; LEAK, DAVID; GOLDBECK, ROSANA. Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes. BIOMASS & BIOENERGY, v. 141, OCT 2020. (17/16159-0, 17/22669-0, 15/50612-8, 19/08542-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BARBOSA, Fernando César. Otimização da produção de celo-oligossacarídeos por hidrólise enzimática de palha de cana-de-açúcar pré-tratada utilizando enzimas celulolíticas e oxidativas. 2020. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.