New analytical strategies to bottom-up and top-down proteomics

• Grant number: 17/17368-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: May 30, 2018
• End date: May 29, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Emanuel Carrilho
• Grantee: Weliton Pedro Batiston
• Supervisor: John Yates III
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Institution abroad: Scripps Research Institute, San Diego, United States
• Associated to the scholarship: 15/16025-8 - Multidimensional chromatographic systems (nano-HPLC and microchip) hyphenated to tandem mass spectrometry of high resolution for quantitative proteomics, BP.DR

Abstract

Currently, the analytical performance to characterize all complexity of a proteome is not sufficient. This complexity has been the main subject of study at Yates Lab for the last 25 years with significant scientific impact on the evolution of the field. With this in mind, we propose a series of studies to try to improve analytical strategies to proteomics, such as i) new chemometrics strategies for optimization of hyphenated analytical techniques; ii) a chemical-mathematical model to increase coverage shotgun proteomics; iii) a thermodynamic equation for stop-flow methods on multidimensional liquid chromatography; iv) the development of new nano-columns to top-down proteomics and v) the development of multidimensional separations on µ-chip. Through Yates Lab expertise, we expect to improve our theories using relevant biological questions in the analytical systems developed, as well as, finding solutions to attacking some of the various limitations of bottom-up and top-down proteomics. (AU)