Characterization of caries-affected dentin and its role on adhesive interfaces

• Grant number: 17/22499-8
• Support type: Scholarships abroad - Research
• Effective date (Start): August 01, 2018
• Effective date (End): July 31, 2019
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Adriana Bona Matos
• Grantee: Adriana Bona Matos
• Host: Ana Karina Barbieri Bedran-Russo
• Home Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Local de pesquisa : University of Illinois at Chicago (UIC), United States

Abstract

This in vitro study aims to characterize and compare natural and artificially obtained caries-affected dentin and to determine its role on hybrid layer formation. In the first phase, in forty human molars natural caries-affected dentin (ICDAS) and artificially obtained by a S. mutans biofilm challenge will be compared to sound dentin. These substrates will be characterized and compared by different methods: collagen maturity (polarized light microscopy), collagen biodegradability (digestibility with collagenase and amino acid analysis) and dentin matrix biomechanics (dynamic mechanical analysis). For the second phase, 24 human molares, containing natural caries affected, artificially obtained dentin will receive and adhesive system as to form hybrid layer. Interfaces will be evaluated by: bond strength (microtensile essay), morphology of the formed hybrid layer (scanning electron microscopy), micro-permeability (confocal microscopy) and in situ zymography (enzyme activity). Qualitative obtained data will be described in details, while quantitative results will be statistically evaluated, accepting 95% of confidence.