The effects of common bean-supplemented diets on gut health, the microbiome and obesity-associated inflammation in mice

• Grant number: 18/03082-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: June 06, 2018
• End date: October 05, 2018
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Marcos Ferreira Minicucci
• Grantee: Bruna Letícia Buzati Pereira
• Supervisor: Krista Anne Power
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: University of Ottawa (uOttawa), Canada
• Associated to the scholarship: 14/23215-5 - Influence of Spondias mombin supplementation on the cardiac remodeling after acute myocardial infarction, BP.DR

Abstract

Obesity is a global epidemic whose hallmarks include systemic and adipose tissue chronic low-grade inflammation, which is driven in part through a dysfunctional intestinal microenvironment. One key strategy to modulate the obese phenotype is the consumption of specific dietary components (e.g. non-digestible carbohydrates (fiber) and phenolics) that are enriched in foods such as common beans. Further, difference between bean varieties, such as seed-coat color, results in dramatic difference in the levels and profiles of phenolic compounds, therefore health effects induced by common beans may be dependent on the common bean variety consumed. This study will determine the effect of bean consumption on aspects of the obese phenotype. Male C57BL/6 mice will consume a high fat diet (HFD) (60% fat as kcal) or an isocaloric HFD supplemented with 15% cooked white beans (HF+WK) or 15% cooked dark-red beans (HF+DK) for 12 weeks. Cooked beans nutritional composition and phenolic compound content, profiles, and antioxidant potential will be determined. Fecal microbial metabolites (phenolics and short chain fatty acid concentrations) will be aseessed, as well as microbial community structure. Colonic, adipose tissue and serum inflammatory and anti-inflammatory cytokines will be measured as well as biomarkers of metabolic function. One-way ANOVA with Fisher's least significant difference posthoc will be used to distinguish differences between group means. The threshold for statistical significance will be p< 0.05.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BUZATI PEREIRA, BRUNA LETICIA; RODRIGUE, ALEXANE; DE OLIVEIRA ARRUDA, FERNANDA CAROLINE; BACHIEGA, TATIANA FERNANDA; MARTINS LOURENCO, MARIA ANGELICA; CORREA, CAMILA RENATA; AZEVEDO, PAULA SCHMIDT; POLEGATO, BERTHA FURLAN; OKOSHI, KATASHI; HENRIQUE FERNANDES, ANA ANGELICA; et al. Spondias mombin L. attenuates ventricular remodelling after myocardial infarction associated with oxidative stress and inflammatory modulation. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, v. 24, n. 14, p. 7862-7872, JUL 2020. (18/03082-1, 15/18502-8, 14/23215-5)

BUZATI PEREIRA, BRUNA LETICIA; RODRIGUE, ALEXANE; DE OLIVEIRA ARRUDA, FERNANDA CAROLINE; BACHIEGA, TATIANA FERNANDA; MARTINS LOURENCO, MARIA ANGELICA; CORREA, CAMILA RENATA; AZEVEDO, PAULA SCHMIDT; POLEGATO, BERTHA FURLAN; OKOSHI, KATASHI; HENRIQUE FERNANDES, ANA ANGELICA; et al. Spondias mombin L. attenuates ventricular remodelling after myocardial infarction associated with oxidative stress and inflammatory modulation. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, MAY 2020. (15/18502-8, 14/23215-5, 18/03082-1)