Several lineages of anurans independently evolved the ability to sequester alkaloids from their diet. To date, there are more than 1400 dietary alkaloids of 24 different classes described for poison frogs, but there is little information about the sequestration mechanism of these compounds from the alkaloid-containing arthropods. Animals that sequester might present means to avoid autointoxication, including modification of compounds either to facilitate excretion or in order to storage in a non-toxic metabolite. Mapping these alkaloids in the body of these amphibians will help elucidate the pathway, and organs and/or tissues that participate in the mechanism of alkaloids and its metabolism. DESI-IMS is a suitable technique for the mapping, since it ionizes the compounds directly in the sample tissue in ambient conditions and requires little preparation of the sample, allowing for better detection of small molecules like alkaloids.
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