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Interaction of cerebellar histaminergic and dopaminergic systems in the modulation of motor and non-motor functions of mice

Grant number: 17/24879-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2018
Effective date (End): August 31, 2022
Field of knowledge:Humanities - Psychology - Physiological Psychology
Principal researcher:Anna Carolyna Lepesteur Gianlorenço
Grantee:Evelyn Maria Guilherme
Home Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The cerebellum is a structure classically related with motor coordination and balance. However, the participation of the cerebellum in non-motor functions has been recently described. The dopaminergic and histaminergic neurotransmitters systems are present in cerebellar neuronal circuit. Evidences suggest that these systems can interact and modulate different functions. Previous studies showed the role of the dopaminergic and histaminergic systems on the modulation of motor performance and motor learning, but the interaction of these systems is still unclear. Therefore, the main objective of the present study is investigate the effects of cerebellar vermis microinjection of histamine and D1 and D2 dopaminergic receptors antagonists, suggesting a possible interaction of these systems and cerebellum in the modulation of motor performance, motor learning and emotional memory of mice. The study will be composed by four experiments, to evaluate de effects of combined injection of histamine and SCH 23390 (dopaminergic D1 receptor antagonist), and combined injection of histamine and sulpiride (dopaminergic D2 receptor antagonist) on motor performance, motor learning and emotional memory in different experimental models, verifying the modulatory effect of dopaminergic system in these functions. For this, a guide cannula with 7 mm in length will be implanted into the cerebellar vermis, through stereotaxic surgery in swiss male mice weighing between 25 and 35 g. Three days after surgery, will be performed the behaviors test, followed by the microinjection procedure, and the same protocol will be repeated 5 minutes, 4 hours and 24 hours later. The histologic analysis will confirm if the injection occurs into the cerebellar vermis, and the statistical analysis will consist at homogeneity test and analysis of variability (ANOVA), followed by appropriated post-hoc. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GUILHERME, EVELYN M.; GIANLORENCO, ANNA CAROLYNA L. The Effects of Intravermis Cerebellar Microinjections of Dopaminergic Agents in Motor Learning and Aversive Memory Acquisition in Mice. FRONTIERS IN BEHAVIORAL NEUROSCIENCE, v. 15, FEB 25 2021. Web of Science Citations: 0.
GUILHERME, EVELYN M.; CARREIRA MOREIRA, ROBERTA DE FATIMA; DE OLIVEIRA, ADRIELE; FERRO, ALYNE MONTERO; PIRES DI LORENZO, VALERIA A.; GIANLORENCO, ANNA CAROLYNA L. Respiratory Disorders in Parkinson's Disease. JOURNAL OF PARKINSONS DISEASE, v. 11, n. 3, p. 993-1010, 2021. Web of Science Citations: 0.

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