The cerebellum is a structure classically related with motor coordination and balance. However, the participation of the cerebellum in non-motor functions has been recently described. The dopaminergic and histaminergic neurotransmitters systems are present in cerebellar neuronal circuit. Evidences suggest that these systems can interact and modulate different functions. Previous studies showed the role of the dopaminergic and histaminergic systems on the modulation of motor performance and motor learning, but the interaction of these systems is still unclear. Therefore, the main objective of the present study is investigate the effects of cerebellar vermis microinjection of histamine and D1 and D2 dopaminergic receptors antagonists, suggesting a possible interaction of these systems and cerebellum in the modulation of motor performance, motor learning and emotional memory of mice. The study will be composed by four experiments, to evaluate de effects of combined injection of histamine and SCH 23390 (dopaminergic D1 receptor antagonist), and combined injection of histamine and sulpiride (dopaminergic D2 receptor antagonist) on motor performance, motor learning and emotional memory in different experimental models, verifying the modulatory effect of dopaminergic system in these functions. For this, a guide cannula with 7 mm in length will be implanted into the cerebellar vermis, through stereotaxic surgery in swiss male mice weighing between 25 and 35 g. Three days after surgery, will be performed the behaviors test, followed by the microinjection procedure, and the same protocol will be repeated 5 minutes, 4 hours and 24 hours later. The histologic analysis will confirm if the injection occurs into the cerebellar vermis, and the statistical analysis will consist at homogeneity test and analysis of variability (ANOVA), followed by appropriated post-hoc. (AU)
News published in Agência FAPESP Newsletter about the scholarship:
GUILHERME, EVELYN M.;
CARREIRA MOREIRA, ROBERTA DE FATIMA;
DE OLIVEIRA, ADRIELE;
FERRO, ALYNE MONTERO;
PIRES DI LORENZO, VALERIA A.;
GIANLORENCO, ANNA CAROLYNA L.
Respiratory Disorders in Parkinson's Disease.
JOURNAL OF PARKINSONS DISEASE,
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